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| | <SX load='6j6i' size='340' side='right' viewer='molstar' caption='[[6j6i]], [[Resolution|resolution]] 3.70Å' scene=''> | | <SX load='6j6i' size='340' side='right' viewer='molstar' caption='[[6j6i]], [[Resolution|resolution]] 3.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6j6i]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6J6I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j6i]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J6I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BQL:[(2~{S})-2-azanyl-3-oxidanylidene-propyl]+[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl+hydrogen+phosphate'>BQL</scene>, <scene name='pdbligand=BQO:[(2~{R},3~{S})-3-azanyl-4-oxidanylidene-butan-2-yl]+[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl+hydrogen+phosphate'>BQO</scene>, <scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PBL2, APK2A, KIN1, At1g14370, F14L17.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), F15B8.100, Resistance related KinaSe 1, RKS1, At3g57710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), RPP13L4, ZAR1, At3g50950, F18B3.230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6i OCA], [https://pdbe.org/6j6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j6i RCSB], [https://www.ebi.ac.uk/pdbsum/6j6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6i ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6j6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6i OCA], [http://pdbe.org/6j6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j6i RCSB], [http://www.ebi.ac.uk/pdbsum/6j6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6i ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PBL2_ARATH PBL2_ARATH]] Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).<ref>PMID:20413097</ref> <ref>PMID:23951354</ref> [[http://www.uniprot.org/uniprot/R13L4_ARATH R13L4_ARATH]] CC-NB-LRR receptor-like protein required for recognition of the Pseudomonas syringae type III effector HopZ1a. Confers allele-specific recognition and virulence attenuation of HopZ1a. Immunity mediated by ZAR1 is independent of several genes required by other resistance protein signaling pathways such as NDR1 and RAR1.<ref>PMID:20368970</ref> | + | [https://www.uniprot.org/uniprot/PBL2_ARATH PBL2_ARATH] Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).<ref>PMID:20413097</ref> <ref>PMID:23951354</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Non-specific serine/threonine protein kinase]]
| + | [[Category: Chai JJ]] |
| - | [[Category: Chai, J J]] | + | [[Category: Hu MJ]] |
| - | [[Category: Hu, M J]] | + | [[Category: Wang HW]] |
| - | [[Category: Wang, H W]] | + | [[Category: Wang J]] |
| - | [[Category: Wang, J]] | + | [[Category: Wang JZ]] |
| - | [[Category: Wang, J Z]] | + | [[Category: Zhou JM]] |
| - | [[Category: Zhou, J M]] | + | |
| - | [[Category: Plant protein]]
| + | |
| - | [[Category: Resistosome]]
| + | |
| Structural highlights
Function
PBL2_ARATH Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).[1] [2]
Publication Abstract from PubMed
Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an alpha-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic alpha helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.
Reconstitution and structure of a plant NLR resistosome conferring immunity.,Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang J, Li W, Xiang T, Liu Z, Laluk K, Ding X, Zou Y, Gao M, Zhang X, Chen S, Mengiste T, Zhang Y, Zhou JM. Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector. Cell Host Microbe. 2010 Apr 22;7(4):290-301. doi: 10.1016/j.chom.2010.03.007. PMID:20413097 doi:http://dx.doi.org/10.1016/j.chom.2010.03.007
- ↑ Guy E, Lautier M, Chabannes M, Roux B, Lauber E, Arlat M, Noel LD. xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK. PLoS One. 2013 Aug 9;8(8):e73469. doi: 10.1371/journal.pone.0073469. eCollection , 2013. PMID:23951354 doi:http://dx.doi.org/10.1371/journal.pone.0073469
- ↑ Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J. Reconstitution and structure of a plant NLR resistosome conferring immunity. Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527 doi:http://dx.doi.org/10.1126/science.aav5870
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