|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4wwi' size='340' side='right'caption='[[4wwi]], [[Resolution|resolution]] 2.31Å' scene=''> | | <StructureSection load='4wwi' size='340' side='right'caption='[[4wwi]], [[Resolution|resolution]] 2.31Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wwi]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wwi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zmd|4zmd]], [[4znc|4znc]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwi OCA], [https://pdbe.org/4wwi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwi RCSB], [https://www.ebi.ac.uk/pdbsum/4wwi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwi ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885]), IGHG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwi OCA], [http://pdbe.org/4wwi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wwi RCSB], [http://www.ebi.ac.uk/pdbsum/4wwi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Deis, L N]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Oas, T G]] | + | [[Category: Deis LN]] |
| - | [[Category: Antibody binding]] | + | [[Category: Oas TG]] |
| - | [[Category: Conformational heterogeneity]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: S. aureus]]
| + | |
| - | [[Category: Three-helix bundle]]
| + | |
| Structural highlights
Function
SPA_STAAU
Publication Abstract from PubMed
Staphylococcal protein A (SpA) is an important virulence factor from Staphylococcus aureus responsible for the bacterium's evasion of the host immune system. SpA includes five small three-helix-bundle domains that can each bind with high affinity to many host proteins such as antibodies. The interaction between a SpA domain and the Fc fragment of IgG was partially elucidated previously in the crystal structure 1FC2. Although informative, the previous structure was not properly folded and left many substantial questions unanswered, such as a detailed description of the tertiary structure of SpA domains in complex with Fc and the structural changes that take place upon binding. Here we report the 2.3-A structure of a fully folded SpA domain in complex with Fc. Our structure indicates that there are extensive structural rearrangements necessary for binding Fc, including a general reduction in SpA conformational heterogeneity, freezing out of polyrotameric interfacial residues, and displacement of a SpA side chain by an Fc side chain in a molecular-recognition pocket. Such a loss of conformational heterogeneity upon formation of the protein-protein interface may occur when SpA binds its multiple binding partners. Suppression of conformational heterogeneity may be an important structural paradigm in functionally plastic proteins.
Suppression of conformational heterogeneity at a protein-protein interface.,Deis LN, Wu Q, Wang Y, Qi Y, Daniels KG, Zhou P, Oas TG Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9028-33. doi:, 10.1073/pnas.1424724112. Epub 2015 Jul 8. PMID:26157136[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Deis LN, Wu Q, Wang Y, Qi Y, Daniels KG, Zhou P, Oas TG. Suppression of conformational heterogeneity at a protein-protein interface. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9028-33. doi:, 10.1073/pnas.1424724112. Epub 2015 Jul 8. PMID:26157136 doi:http://dx.doi.org/10.1073/pnas.1424724112
|
