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| | <StructureSection load='4wwu' size='340' side='right'caption='[[4wwu]], [[Resolution|resolution]] 3.30Å' scene=''> | | <StructureSection load='4wwu' size='340' side='right'caption='[[4wwu]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wwu]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wwu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MEX67, YPL169C, P2520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), MTR2, YKL186C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwu OCA], [https://pdbe.org/4wwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwu RCSB], [https://www.ebi.ac.uk/pdbsum/4wwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwu OCA], [http://pdbe.org/4wwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wwu RCSB], [http://www.ebi.ac.uk/pdbsum/4wwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST]] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref> [[http://www.uniprot.org/uniprot/MTR2_YEAST MTR2_YEAST]] Affects mRNA transport from the nucleus to the cytoplasm. | + | [https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aibara, S]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Stewart, M]] | + | [[Category: Aibara S]] |
| - | [[Category: Valkov, E]] | + | [[Category: Stewart M]] |
| - | [[Category: Mrna nuclear export factor]] | + | [[Category: Valkov E]] |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
MEX67_YEAST Involved in the export of mRNA from the nucleus to the cytoplasm.[1]
Publication Abstract from PubMed
The Mex67:Mtr2 complex is the principal yeast nuclear export factor for bulk mRNA and also contributes to ribosomal subunit export. Mex67 is a modular protein constructed from four domains (RRM, LRR, NTF2-like and UBA) that have been thought to be joined by flexible linkers like beads on a string, with the RRM and LRR domains binding RNAs and the NTF2-like and UBA domains binding FG-nucleoporins to facilitate movement through nuclear pores. Here, we show that the NTF2-like domain from Saccharomyces cerevisiae Mex67:Mtr2 also contributes to RNA binding. Moreover, the 3.3 A resolution crystal structure of the Mex67DeltaUBA:Mtr2 complex, supplemented with small angle X-ray scattering data, indicated that the LRR domain has a defined spatial relationship to the Mex67NTF2L:Mtr2 region. Conversely, the RRM domain and especially the UBA domain are more mobile. The conformation assumed by the LRR and NTF2-like domains results in clusters of positively-charged residues on each becoming arranged to form a continuous interface for binding RNA on the opposite side of the complex to the region that interacts with FG-nucleoporins to facilitate passage through nuclear pores.
Domain organization within the nuclear export factor Mex67:Mtr2 generates an extended mRNA binding surface.,Aibara S, Valkov E, Lamers M, Stewart M Nucleic Acids Res. 2015 Jan 23. pii: gkv030. PMID:25618852[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Segref A, Sharma K, Doye V, Hellwig A, Huber J, Luhrmann R, Hurt E. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J. 1997 Jun 2;16(11):3256-71. PMID:9214641 doi:http://dx.doi.org/10.1093/emboj/16.11.3256
- ↑ Aibara S, Valkov E, Lamers M, Stewart M. Domain organization within the nuclear export factor Mex67:Mtr2 generates an extended mRNA binding surface. Nucleic Acids Res. 2015 Jan 23. pii: gkv030. PMID:25618852 doi:http://dx.doi.org/10.1093/nar/gkv030
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