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| | <StructureSection load='4wyh' size='340' side='right'caption='[[4wyh]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='4wyh' size='340' side='right'caption='[[4wyh]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WYH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSO0502 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [https://pdbe.org/4wyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [https://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://pdbe.org/4wyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q97ZS7_SACS2 Q97ZS7_SACS2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sacs2]] | + | [[Category: Saccharolobus solfataricus P2]] |
| - | [[Category: Ouyang, S]] | + | [[Category: Ouyang S]] |
| - | [[Category: A novel noncatalytic subunit archaeal eukaryal-type primase]]
| + | |
| - | [[Category: Replication]]
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| Structural highlights
Function
Q97ZS7_SACS2
Publication Abstract from PubMed
Archaea encode a eukaryotic-type primase comprising a catalytic subunit (PriS) and a noncatalytic subunit (PriL). Here we report the identification of a primase noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus. Like PriL, PriX is essential for the survival of the organism. The crystallographic analysis complemented by sensitive sequence comparisons shows that PriX is a diverged homologue of the C-terminal domain of PriL but lacks the iron-sulfur cluster. Phylogenomic analysis provides clues on the origin and evolution of PriX. PriX, PriL and PriS form a stable heterotrimer (PriSLX). Both PriSX and PriSLX show far greater affinity for nucleotide substrates and are substantially more active in primer synthesis than the PriSL heterodimer. In addition, PriL, but not PriX, facilitates primer extension by PriS. We propose that the catalytic activity of PriS is modulated through concerted interactions with the two noncatalytic subunits in primer synthesis.
A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase.,Liu B, Ouyang S, Makarova KS, Xia Q, Zhu Y, Li Z, Guo L, Koonin EV, Liu ZJ, Huang L Nat Commun. 2015 Jun 22;6:7300. doi: 10.1038/ncomms8300. PMID:26095544[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Liu B, Ouyang S, Makarova KS, Xia Q, Zhu Y, Li Z, Guo L, Koonin EV, Liu ZJ, Huang L. A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase. Nat Commun. 2015 Jun 22;6:7300. doi: 10.1038/ncomms8300. PMID:26095544 doi:http://dx.doi.org/10.1038/ncomms8300
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