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| | <StructureSection load='4wzs' size='340' side='right'caption='[[4wzs]], [[Resolution|resolution]] 3.78Å' scene=''> | | <StructureSection load='4wzs' size='340' side='right'caption='[[4wzs]], [[Resolution|resolution]] 3.78Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wzs]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn] and [http://en.wikipedia.org/wiki/Enccu Enccu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WZS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wzs]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi], [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi_GB-M1 Encephalitozoon cuniculi GB-M1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WZS FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECU11_1470 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284813 ENCCU]), ECU11_0730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN]), ECU03_1530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284813 ENCCU]), ECU04_1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284813 ENCCU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wzs OCA], [https://pdbe.org/4wzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wzs RCSB], [https://www.ebi.ac.uk/pdbsum/4wzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wzs ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wzs OCA], [http://pdbe.org/4wzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wzs RCSB], [http://www.ebi.ac.uk/pdbsum/4wzs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wzs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8SQT6_ENCCU Q8SQT6_ENCCU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Enccn]] | + | [[Category: Encephalitozoon cuniculi]] |
| - | [[Category: Enccu]] | + | [[Category: Encephalitozoon cuniculi GB-M1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Butryn, A]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Hopfner, K P]] | + | [[Category: Butryn A]] |
| - | [[Category: Protein-dna complex]] | + | [[Category: Hopfner K-P]] |
| - | [[Category: Swi2/snf2]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
Q8SQT6_ENCCU
Publication Abstract from PubMed
Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 dissociates TATA box-binding protein (TBP):DNA complexes, offering a useful system to address the structural mechanisms of Swi2/Snf2 ATPases. Here we report the crystal structure of the N-terminal domain of Mot1 in complex with TBP, DNA, and the transcription regulator NC2. Our data show that Mot1 reduces DNA:NC2 interactions and unbends DNA as compared to the TBP:DNA:NC2 state, suggesting that Mot1 primes TBP:NC2 displacement in an ATP-independent manner. Electron microscopy and cross-linking data suggest that the Swi2/Snf2 domain of Mot1 associates with the upstream DNA and the histone fold of NC2, thereby revealing parallels to some nucleosome remodelers. This study provides a structural framework for how a Swi2/Snf2 ATPase interacts with its substrate DNA:protein complex.
Structural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1.,Butryn A, Schuller JM, Stoehr G, Runge-Wollmann P, Forster F, Auble DT, Hopfner KP Elife. 2015 Aug 10;4. doi: 10.7554/eLife.07432. PMID:26258880[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Butryn A, Schuller JM, Stoehr G, Runge-Wollmann P, Forster F, Auble DT, Hopfner KP. Structural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1. Elife. 2015 Aug 10;4. doi: 10.7554/eLife.07432. PMID:26258880 doi:http://dx.doi.org/10.7554/eLife.07432
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