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| | ==Fatty Acid and Retinol binding protein Na-FAR-1 from Necator americanus== | | ==Fatty Acid and Retinol binding protein Na-FAR-1 from Necator americanus== |
| - | <StructureSection load='4xcp' size='340' side='right' caption='[[4xcp]], [[Resolution|resolution]] 2.14Å' scene=''> | + | <StructureSection load='4xcp' size='340' side='right'caption='[[4xcp]], [[Resolution|resolution]] 2.14Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_hookworm Human hookworm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XCP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xcp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XCP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xcp OCA], [https://pdbe.org/4xcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xcp RCSB], [https://www.ebi.ac.uk/pdbsum/4xcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xcp ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NECAME_14208 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=51031 Human hookworm])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xcp OCA], [http://pdbe.org/4xcp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xcp RCSB], [http://www.ebi.ac.uk/pdbsum/4xcp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xcp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/W2SRJ3_NECAM W2SRJ3_NECAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4xcp" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4xcp" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Retinol-binding protein 3D structures|Retinol-binding protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human hookworm]] | + | [[Category: Large Structures]] |
| - | [[Category: Corsico, B]] | + | [[Category: Necator americanus]] |
| - | [[Category: Gabrielsen, M]] | + | [[Category: Corsico B]] |
| - | [[Category: Griffiths, K]] | + | [[Category: Gabrielsen M]] |
| - | [[Category: Ibanez-Shimabukuro, M]] | + | [[Category: Griffiths K]] |
| - | [[Category: Kennedy, M W]] | + | [[Category: Ibanez-Shimabukuro M]] |
| - | [[Category: Rey-Burusco, M F]] | + | [[Category: Kennedy MW]] |
| - | [[Category: Smith, B O]] | + | [[Category: Rey-Burusco MF]] |
| - | [[Category: Fatty acid retinol binding]]
| + | [[Category: Smith BO]] |
| - | [[Category: Retinol-binding protein]]
| + | |
| Structural highlights
Function
W2SRJ3_NECAM
Publication Abstract from PubMed
Fatty acid and retinol-binding proteins (FARs) comprise a family of unusual alpha-helix rich lipid-binding proteins found exclusively in nematodes. They are secreted into host tissues by parasites of plants, animals and humans. The structure of a FAR protein from the free-living nematode Caenorhabditis elegans is available, but this protein [C. elegans FAR-7 (Ce-FAR-7)] is from a subfamily of FARs that does not appear to be important at the host/parasite interface. We have therefore examined [Necator americanus FAR-1 (Na-FAR-1)] from the blood-feeding intestinal parasite of humans, N. americanus. The 3D structure of Na-FAR-1 in its ligand-free and ligand-bound forms, determined by NMR (nuclear magnetic resonance) spectroscopy and X-ray crystallography respectively, reveals an alpha-helical fold similar to Ce-FAR-7, but Na-FAR-1 possesses a larger and more complex internal ligand-binding cavity and an additional C-terminal alpha-helix. Titration of apo-Na-FAR-1 with oleic acid, analysed by NMR chemical shift perturbation, reveals that at least four distinct protein-ligand complexes can be formed. Na-FAR-1 and possibly other FARs may have a wider repertoire for hydrophobic ligand binding, as confirmed in the present study by our finding that a range of neutral and polar lipids co-purify with the bacterially expressed recombinant protein. Finally, we show by immunohistochemistry that Na-FAR-1 is present in adult worms with a tissue distribution indicative of possible roles in nutrient acquisition by the parasite and in reproduction in the male.
Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus.,Rey-Burusco MF, Ibanez-Shimabukuro M, Gabrielsen M, Franchini GR, Roe AJ, Griffiths K, Zhan B, Cooper A, Kennedy MW, Corsico B, Smith BO Biochem J. 2015 Nov 1;471(3):403-14. doi: 10.1042/BJ20150068. Epub 2015 Aug 28. PMID:26318523[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rey-Burusco MF, Ibanez-Shimabukuro M, Gabrielsen M, Franchini GR, Roe AJ, Griffiths K, Zhan B, Cooper A, Kennedy MW, Corsico B, Smith BO. Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus. Biochem J. 2015 Nov 1;471(3):403-14. doi: 10.1042/BJ20150068. Epub 2015 Aug 28. PMID:26318523 doi:http://dx.doi.org/10.1042/BJ20150068
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