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| | <StructureSection load='4xd9' size='340' side='right'caption='[[4xd9]], [[Resolution|resolution]] 2.35Å' scene=''> | | <StructureSection load='4xd9' size='340' side='right'caption='[[4xd9]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xd9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XD9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xd9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XD9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AN3745.2, ANIA_03745 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), ANIA_10200 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xd9 OCA], [https://pdbe.org/4xd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xd9 RCSB], [https://www.ebi.ac.uk/pdbsum/4xd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xd9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xd9 OCA], [http://pdbe.org/4xd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xd9 RCSB], [http://www.ebi.ac.uk/pdbsum/4xd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xd9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C8VMF9_EMENI C8VMF9_EMENI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspergillus nidulans]] | + | [[Category: Aspergillus nidulans FGSC A4]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Asano, N]] | + | [[Category: Asano N]] |
| - | [[Category: Kato, K]] | + | [[Category: Kato K]] |
| - | [[Category: Yao, M]] | + | [[Category: Yao M]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Translation]]
| + | |
| Structural highlights
Function
C8VMF9_EMENI
Publication Abstract from PubMed
Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. These proteins are necessary for the recruitment of three ribosomal components (5S ribosomal RNA [rRNA], RpL5 and RpL11) to the 90S ribosome precursor and subsequent 27SB pre-rRNA processing. Here we present the crystal structure of the Aspergillus nidulans (An) Rpf2-Rrs1 core complex. The core complex contains the tightly interlocked N-terminal domains of Rpf2 and Rrs1. The Rpf2 N-terminal domain includes a Brix domain characterized by similar N- and C-terminal architecture. The long alpha-helix of Rrs1 joins the C-terminal half of the Brix domain as if it were part of a single molecule. The conserved proline-rich linker connecting the N- and C-terminal domains of Rrs1 wrap around the side of Rpf2 and anchor the C-terminal domain of Rrs1 to a specific site on Rpf2. In addition, gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA. Further analysis of Rpf2-Rrs1 mutants demonstrated that Saccharomyces cerevisiae Rpf2 R236 (corresponds to R238 of AnRpf2) plays a significant role in this binding. Based on these studies and previous reports, we have proposed a model for ribosomal component recruitment to the 90S ribosome precursor.
Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.,Asano N, Kato K, Nakamura A, Komoda K, Tanaka I, Yao M Nucleic Acids Res. 2015 May 19;43(9):4746-57. doi: 10.1093/nar/gkv305. Epub 2015 , Apr 8. PMID:25855814[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Asano N, Kato K, Nakamura A, Komoda K, Tanaka I, Yao M. Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis. Nucleic Acids Res. 2015 May 19;43(9):4746-57. doi: 10.1093/nar/gkv305. Epub 2015 , Apr 8. PMID:25855814 doi:http://dx.doi.org/10.1093/nar/gkv305
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