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| | <StructureSection load='4xgy' size='340' side='right'caption='[[4xgy]], [[Resolution|resolution]] 1.49Å' scene=''> | | <StructureSection load='4xgy' size='340' side='right'caption='[[4xgy]], [[Resolution|resolution]] 1.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XGY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XGY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgy OCA], [https://pdbe.org/4xgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xgy RCSB], [https://www.ebi.ac.uk/pdbsum/4xgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgy ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2b3p|2b3p]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgy OCA], [http://pdbe.org/4xgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xgy RCSB], [http://www.ebi.ac.uk/pdbsum/4xgy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | + | [https://www.uniprot.org/uniprot/A0A059PIQ0_AEQVI A0A059PIQ0_AEQVI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aeqvi]] | + | [[Category: Aequorea victoria]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, Y G]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Shi, N]] | + | [[Category: Vibrio parahaemolyticus]] |
| - | [[Category: Wang, S H]] | + | [[Category: Chen YG]] |
| - | [[Category: Cdr3]] | + | [[Category: Shi N]] |
| - | [[Category: Fluorescent protein]] | + | [[Category: Wang SH]] |
| - | [[Category: Fluorobody]]
| + | |
| - | [[Category: Gfp]]
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| Structural highlights
Function
A0A059PIQ0_AEQVI
Publication Abstract from PubMed
A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacterium Vibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space group P43212, with unit-cell parameters a = b = 63.35, c = 125.90 A, were obtained by vapour diffusion in hanging drops and the structure was refined to an Rfree of 16.7% at 1.5 A resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.
The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.,Chen Y, Huang X, Wang R, Wang S, Shi N Acta Crystallogr F Struct Biol Commun. 2015 Jul;71(Pt 7):913-8. doi:, 10.1107/S2053230X15008845. Epub 2015 Jun 27. PMID:26144238[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chen Y, Huang X, Wang R, Wang S, Shi N. The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus. Acta Crystallogr F Struct Biol Commun. 2015 Jul;71(Pt 7):913-8. doi:, 10.1107/S2053230X15008845. Epub 2015 Jun 27. PMID:26144238 doi:http://dx.doi.org/10.1107/S2053230X15008845
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