Introduction

The adaptive immune response possessed by vertebrate animals owes much of its function to B cells. These specialized immune cells produce antibodies and Immunoglobulins (Ig), the membrane bound equivalent to antibodies. B cells can produce a variety of Ig compounds including IgG, IgA, IgE, IgD, and IgM. These antibodies and Ig compounds bind to specific compounds called antigens. When an IgM combines with a B cell receptor (BCR) it can then send a signal in the form of a conformational change through the B cell membrane to stimulate the production of more antibodies that recognize that antigen. ^[1]

The structure of the IgM BCR complex was determined by two research groups using Cryo EM. They also determined the structure of IgG. ^[2], ^[3]

Structure

Figure 1. IgM BCR Structure Overview. Depiction of the IgM BCR expressed on the membrane of a B cell. Includes all major components including the α/β heterodimer, heavy and light chains, antigen binding sites, and the ITAM region for signal transduction.

Transmembrane Region

The IgM BCR is anchored to B-cell membranes through the which is broken up into both extracellular and integral domains which sit on top of or span through the membrane, respectively (Figure 1). IgM BCR assembly requires dimerization of the Igα and Igβ subunits which embed within the B-cell membrane. ^[4] The dimerizes within the extracellular region with a . Additional dimerization is believed to occur within the integral region via a hydrogen bond; the involved residues and interaction have not been confirmed. Although the mechanism of disulfide bridge formation is still unknown, it is believed that via N-linked asparagine glycosylation (NAGs) on various residues in the extracellular region of both the Igα and and Igβ chains help facilitate this process. Chaperone proteins are typically bound to the alpha and beta subunits until dimerization occurs; at this point the rest of the BCR complex can be recruited. ^[5]

After Igα and Igβ dimerization, the transmembrane helices of the heavy chains can embed within the B-cell membrane. ^[4] The side chains of this are primarily hydrophobic side chains that allow for interactions with the hydrophobic tails in the phospholipid bilayer. The four helices (Figure 2) are primarily held together through hydrophobic interactions; however, a a few polar residues are included on the interior of the helix structure which interact with a few polar residues on the Igα and Igβ chains. ^[5]

Figure 2. 4-pass integral helix. Pymol image of the integral helices in IgM BCR (PDB:7xq8) rotated on the x and y axes. Side chains are shown as sticks. Brown=Ig alpha, orange=Ig beta, pink=heavy chain A, blue=heavy chain B.

Within the transmembrane region, heavy chain A and heavy chain B associate (Figure 1) asymmetrically to facilitate intracellular signaling cascades. The transmembrane heavy chain interface allows them to pack together via Van der Waals contacts, but there are also prominent between each chain. More specifically, the hydroxyl group from Ser584 on heavy chain A donates a hydrogen bond to Ser584 and to Ser588 on heavy chain B. This creates a bifurcated hydrogen bond, essentially forming a “fork” between the two chains to help stabilize them and maintain the transmission of the signal once the cell is activated. Because transmembrane Ig molecules cannot efficiently initiate the signal cascade, they must associate with the Igα and Igβ chains within the BCR. ^[2]

Furthermore, both the Igα and Igβ chains have cytoplasmic tails that extend into the B cell (Figure 1). Each of these tails contain an ITAM region to facilitate signal transduction. However, the structural details about these regions are unknown due to a lack of visualization of the tails, thus cannot be conserved in the protein structure (Figure 4). ^[3]

Fc Region

The constant region of IgM is made up of the two . These heavy chains form a bridge connecting the FAB region or variable region to the transmembrane region (Figure 1). They also act as a wire that the variable region can send a signal through to the transmembrane region as a mechanical change.

help hold the heavy chains and Igα/Igβ chains together in the extracellular portion of the transmembrane region.

Because a conformational change occurs throughout the entirety of the IgM-BCR complex, the Fc region must be able to tolerate the contortion of the molecule as the antigen binds. In constant region two, which is located at the start of the Fc region, heavy chain A and heavy chain B make a to stabilize the IgM-BCR and drive downstream signaling.

To maximize the Fc region’s signal transduction efficiency and Van der Waals contacts, constant region two of heavy chain A makes an asymmetrical association with constant region three of heavy chain B to create a stabilizing . More specifically, Arg243 and Arg251 residues from heavy chain A donate three hydrogen bonds to Leu433, Thr431, and Asp376 residues on heavy chain B. Furthermore, Leu313 of heavy chain A accepts a hydrogen bond from Thr429 on heavy chain B. By packing together this closely, it allows the IgM antibody to transmit extracellular stimuli, upon an antigen binding, to the intracellular region to facilitate its biological functions. ^[3]

Fab Region

The of the antibody is where antigen recognition occurs upon binding (Figure 1). On each arm is one heavy (A/B) and one light (A/B) chain, both containing domains identical to their respective counterparts. Repeats of β-sandwiches form the constant and variable domains within the Fab region as antigen recognition occurs at the variable domain while the constant domain connects it to the rest of the IgM complex. Because the Fab region of IgM is poorly resolved, a structural analysis of an HIV neutralizing antibody called VCR01 was performed to approximate where an antigen would bind to at the . ^[6] The IgM-BCR contains areas referred to as complementary-determining regions(CDRs), which are where the antigen makes with the antibody on the Fab domain. Figure 2 depicts this as a surface representation given that the specific residues within the antigen-binding motif are unknown.

Due to the poor resolution of the Fab region, specific side chain interactions between the heavy (A/B) and light (A/B) chains have not been determined. Based on each β-sandwich, it is estimated that they each contain one disulfide bridge with additional hydrogen bonds. The poor resolution does not allow them to be visualized within the structure, but upon understanding the significance of cellular signals being transmitted down to the Fc region, the presence of disulfide bonds would allow the protein to maintain its stability during conformational changes that occur as a result of signal modulation. The shows how the four heavy and light chain β-sandwiches fit together. The Fab region heavy chains attach to the Fc region heavy chains, before continuing down into the intracellular domain to interact with the Igα/Igβ subunits. The light chains (A/B) however are only connected to the heavy chains (A/B) within the Fab region, thus have no contact with the Igα/Igβ heterodimer.

Figure 3. Surface Representation of IgM Antibody Binding Pocket. On one arm of the IgM antibody, the antigen makes contact with light chain A at the L1 and L3 complementary-determining regions. The antigen also makes contact with heavy chain A at the H1, H2, and H3 complementary-determining regions. The location of the complementary-determining regions were approximated using the structure of the VCR01 variable region and were visualized using Pymol.

Signal Transduction

Based on the structure of IgM, the diagram in Figure 4 depicts the initial process of B cell activation by the antigen binding to the antibody at the Fab region. The underlying mechanism for signal transduction is unknown due to the lack of specificity on which tyrosine residues in the Igα and Igβ domains are phosphorylated, but it is speculated to operate based on the conserved assembly mechanism. ^[3] Following an antigen binding, BCRs on the surface of the cell begin to cluster to cause the phosphorylation of the ITAMs located in Igα and Igβ. In its “off” state, the constant region 4 of heavy chain B the extracellular components of Igα and Igβ. The antigen binding induces a conformational change to release the overlap and allow for clustering about the BCR. Now, in its “on” state, the phosphorylation of the ITAM region within the intracellular tails of Igα and Igβ drives downstream kinase activity. As observed in Figure 4, the activated antibody can now continue to process of intracellular signal cascading.

Figure 4. IgM Antibody Signal Transduction following Antigen Binding. The tyrosine residues on the intracellular end of Igα and Igβ helices are phosphorylated by potential tyrosine kinases: Splenic-tyrosine kinase and Src family kinase. Although the exact mechanism is unknown, the phosphorylation of Igα and Igβ activates the B cell and triggers intracellular downstream signaling.