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1kmh
From Proteopedia
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[[Image:1kmh.gif|left|200px]] | [[Image:1kmh.gif|left|200px]] | ||
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'''Crystal Structure of spinach chloroplast F1-ATPase complexed with tentoxin''' | '''Crystal Structure of spinach chloroplast F1-ATPase complexed with tentoxin''' | ||
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==Reference== | ==Reference== | ||
Structure of spinach chloroplast F1-ATPase complexed with the phytopathogenic inhibitor tentoxin., Groth G, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3464-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11904410 11904410] | Structure of spinach chloroplast F1-ATPase complexed with the phytopathogenic inhibitor tentoxin., Groth G, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3464-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11904410 11904410] | ||
| - | [[Category: H(+)-transporting two-sector ATPase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Spinacia oleracea]] | [[Category: Spinacia oleracea]] | ||
[[Category: Groth, G.]] | [[Category: Groth, G.]] | ||
| - | [[Category: | + | [[Category: Protein-inhibitor complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:54:59 2008'' | |
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Revision as of 19:55, 2 May 2008
Crystal Structure of spinach chloroplast F1-ATPase complexed with tentoxin
Overview
Tentoxin, a natural cyclic tetrapeptide produced by phytopathogenic fungi from the Alternaria species affects the catalytic function of the chloroplast F(1)-ATPase in certain sensitive species of plants. In this study, we show that the uncompetitive inhibitor tentoxin binds to the alphabeta-interface of the chloroplast F(1)-ATPase in a cleft localized at betaAsp-83. Most of the binding site is located on the noncatalytic alpha-subunit. The crystal structure of the tentoxin-inhibited CF(1)-complex suggests that the inhibitor is hydrogen bonded to Asp-83 in the catalytic beta-subunit but forms hydrophobic contacts with residues Ile-63, Leu-65, Val-75, Tyr-237, Leu-238, and Met-274 in the adjacent alpha-subunit. Except for minor changes around the tentoxin-binding site, the structure of the chloroplast alpha(3)beta(3)-core complex is the same as that determined with the native chloroplast ATPase. Tentoxin seems to act by inhibiting inter-subunit contacts at the alphabeta-interface and by blocking the interconversion of binding sites in the catalytic mechanism.
About this Structure
1KMH is a Protein complex structure of sequences from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Structure of spinach chloroplast F1-ATPase complexed with the phytopathogenic inhibitor tentoxin., Groth G, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3464-8. PMID:11904410 Page seeded by OCA on Fri May 2 22:54:59 2008
