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Function of Lactonase Enzyme

The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.

Biological Relevance and Broader Implications

Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.

Important Amino Acids

The first five amino acids of the amino terminus at physiological pH are MSNVR.

Structural Highlights

Secondary Structure: Consists os six blades of B-propeller, phosphate molecule bound to calcium and sodium ion with a helix attached to it. Tertiary Structure: Calcium coordination sphere shows the contacts holding the calcium ion in place. Quaternary structure: Carbonyl oxygen of 2-HQ overlaps with one of the phosphate oxygens.