1kmn

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[[Image:1kmn.gif|left|200px]]
[[Image:1kmn.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1kmn |SIZE=350|CAPTION= <scene name='initialview01'>1kmn</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_1kmn", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=S1A:HIS+And+Atp+Binding+Site,+Substrates+Of+First+Reaction+H+...'>S1A</scene>, <scene name='pdbsite=S1B:HIS+And+Atp+Binding+Site,+Substrates+Of+First+Reaction+H+...'>S1B</scene>, <scene name='pdbsite=S1C:HIS+And+Atp+Binding+Site,+Substrates+Of+First+Reaction+H+...'>S1C</scene> and <scene name='pdbsite=S1D:HIS+And+Atp+Binding+Site,+Substrates+Of+First+Reaction+H+...'>S1D</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=HSO:HISTIDINOL'>HSO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1kmn| PDB=1kmn | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kmn OCA], [http://www.ebi.ac.uk/pdbsum/1kmn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kmn RCSB]</span>
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}}
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'''HISTIDYL-TRNA SYNTHETASE COMPLEXED WITH HISTIDINOL AND ATP'''
'''HISTIDYL-TRNA SYNTHETASE COMPLEXED WITH HISTIDINOL AND ATP'''
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[[Category: Francklyn, C S.]]
[[Category: Francklyn, C S.]]
[[Category: Moras, D.]]
[[Category: Moras, D.]]
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[[Category: aminoacyl-trna synthase]]
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[[Category: Aminoacyl-trna synthase]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: synthetase]]
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[[Category: Synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:55:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:50:41 2008''
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Revision as of 19:55, 2 May 2008

Image:1kmn.gif

Template:STRUCTURE 1kmn

HISTIDYL-TRNA SYNTHETASE COMPLEXED WITH HISTIDINOL AND ATP


Overview

The crystal structure of an enzyme-substrate complex with histidyl-tRNA synthetase from Escherichia coli, ATP, and the amino acid analog histidinol is described and compared with the previously obtained enzyme-product complex with histidyl-adenylate. An active site arginine, Arg-259, unique to all histidyl-tRNA synthetases, plays the role of the catalytic magnesium ion seen in seryl-tRNA synthetase. When Arg-259 is substituted with histidine, the apparent second order rate constant (kcat/Km) for the pyrophosphate exchange reaction and the aminoacylation reaction decreases 1,000-fold and 500-fold, respectively. Crystals soaked with MnCl2 reveal the existence of two metal binding sites between beta- and gamma-phosphates; these sites appear to stabilize the conformation of the pyrophosphate. The use of both conserved metal ions and arginine in phosphoryl transfer provides evidence of significant early functional divergence of class II aminoacyl-tRNA synthetases.

About this Structure

1KMN is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase., Arnez JG, Augustine JG, Moras D, Francklyn CS, Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7144-9. PMID:9207058 Page seeded by OCA on Fri May 2 22:55:16 2008

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OCA

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