8cjh

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'''Unreleased structure'''
 
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The entry 8cjh is ON HOLD until Paper Publication
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==Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin==
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<StructureSection load='8cjh' size='340' side='right'caption='[[8cjh]], [[Resolution|resolution]] 2.98&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8cjh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CJH FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cjh OCA], [https://pdbe.org/8cjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cjh RCSB], [https://www.ebi.ac.uk/pdbsum/8cjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cjh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q0P7K1_ECOLX Q0P7K1_ECOLX]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The genotoxin colibactin produced by Escherichia coli is involved in the development of colorectal cancers. This secondary metabolite is synthesized by a multi-protein machinery, mainly composed of non-ribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) enzymes. In order to decipher the function of a PKS-NRPS hybrid enzyme implicated in a key step of colibactin biosynthesis, we conducted an extensive structural characterization of the ClbK megaenzyme. Here we present the crystal structure of the complete trans-AT PKS module of ClbK showing structural specificities of hybrid enzymes. In addition, we report the SAXS solution structure of the full-length ClbK hybrid that reveals a dimeric organization as well as several catalytic chambers. These results provide a structural framework for the transfer of a colibactin precursor through a PKS-NRPS hybrid enzyme and can pave the way for re-engineering PKS-NRPS hybrid megaenzymes to generate diverse metabolites with many applications.
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Authors: Bonhomme, S., Dessen, A., Macheboeuf, P.
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Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin.,Bonhomme S, Contreras-Martel C, Dessen A, Macheboeuf P Structure. 2023 Apr 4:S0969-2126(23)00095-3. doi: 10.1016/j.str.2023.03.012. PMID:37059096<ref>PMID:37059096</ref>
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Description: Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Macheboeuf, P]]
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<div class="pdbe-citations 8cjh" style="background-color:#fffaf0;"></div>
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[[Category: Dessen, A]]
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== References ==
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[[Category: Bonhomme, S]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Bonhomme S]]
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[[Category: Dessen A]]
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[[Category: Macheboeuf P]]

Revision as of 17:04, 26 April 2023

Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin

PDB ID 8cjh

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