1jv3
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(New page: 200px<br /> <applet load="1jv3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jv3, resolution 2.20Å" /> '''CRYSTAL STRUCTURE O...)
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Revision as of 15:38, 12 November 2007
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CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGALNAC
Overview
The recently published human genome with its relatively modest number of, genes has highlighted the importance of post-transcriptional and, post-translational modifications, such as alternative splicing or, glycosylation, in generating the complexities of human biology. The human, UDP-N-acetylglucosamine (UDPGlcNAc) pyrophosphorylases AGX1 and AGX2, which differ in sequence by an alternatively spliced 17 residue peptide, are key enzymes synthesizing UDPGlcNAc, an essential precursor for protein, glycosylation. To better understand the catalytic mechanism of these, enzymes and the role of the alternatively spliced segment, we have solved, the crystal structures of AGX1 and AGX2 in complexes with UDPGlcNAc (at, 1.9 and 2.4 A resolution, respectively) and UDPGalNAc (at 2.2 and 2.3 A, resolution, respectively). Comparison with known structures classifies, AGX1 and AGX2 as two new members of the SpsA-GnT I Core superfamily and, together with mutagenesis analysis, helps identify residues critical for, catalysis. Most importantly, our combined structural and biochemical data, provide evidence for a change in the oligomeric assembly accompanied by a, significant modification of the active site architecture, a result, suggesting that the two isoforms generated by alternative splicing may, have distinct catalytic properties.
About this Structure
1JV3 is a Single protein structure of sequence from Homo sapiens with UD2 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structures of two human pyrophosphorylase isoforms in complexes with UDPGlc(Gal)NAc: role of the alternatively spliced insert in the enzyme oligomeric assembly and active site architecture., Peneff C, Ferrari P, Charrier V, Taburet Y, Monnier C, Zamboni V, Winter J, Harnois M, Fassy F, Bourne Y, EMBO J. 2001 Nov 15;20(22):6191-202. PMID:11707391
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