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| | ==X-ray structure of bGFP-C / EGFP complex== | | ==X-ray structure of bGFP-C / EGFP complex== |
| - | <StructureSection load='4xvp' size='340' side='right' caption='[[4xvp]], [[Resolution|resolution]] 3.40Å' scene=''> | + | <StructureSection load='4xvp' size='340' side='right'caption='[[4xvp]], [[Resolution|resolution]] 3.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xvp]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XVP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XVP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xvp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XVP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XVP FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xl5|4xl5]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xvp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xvp OCA], [https://pdbe.org/4xvp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xvp RCSB], [https://www.ebi.ac.uk/pdbsum/4xvp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xvp ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xvp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xvp OCA], [http://pdbe.org/4xvp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xvp RCSB], [http://www.ebi.ac.uk/pdbsum/4xvp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xvp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | + | [https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4xvp" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4xvp" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aeqvi]] | + | [[Category: Aequorea victoria]] |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Large Structures]] |
| - | [[Category: Chevrel, A]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Minard, P]] | + | [[Category: Chevrel A]] |
| - | [[Category: Sierra-Gallay, I Li de la]] | + | [[Category: Li de la Sierra-Gallay I]] |
| - | [[Category: Tilbeurgh, H Van]] | + | [[Category: Minard P]] |
| - | [[Category: Urvoas, A]] | + | [[Category: Urvoas A]] |
| - | [[Category: Valerio-Lepiniec, M]] | + | [[Category: Valerio-Lepiniec M]] |
| - | [[Category: Alpharep scaffold]] | + | [[Category: Van Tilbeurgh H]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Egfp]]
| + | |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Heat-like repeat]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Protein engineering]]
| + | |
| Structural highlights
Function
GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Publication Abstract from PubMed
A family of artificial proteins, named alphaRep, based on a natural family of helical repeat was previously designed. alphaRep members are efficiently expressed, folded and extremely stable proteins. A large alphaRep library was constructed creating proteins with a randomized interaction surface. In the present study, we show that the alphaRep library is an efficient source of tailor-made specific proteins with direct applications in biochemistry and cell biology. From this library, we selected by phage display alphaRep binders with nanomolar dissociation constants against the GFP. The structures of two independent alphaRep binders in complex with the GFP target were solved by X-ray crystallography revealing two totally different binding modes. The affinity of the selected alphaReps for GFP proved sufficient for practically useful applications such as pull-down experiments. alphaReps are disulfide free proteins and are efficiently and functionally expressed in eukaryotic cells: GFP-specific alphaReps are clearly sequestrated by their cognate target protein addressed to various cell compartments. These results suggest that alphaRep proteins with tailor-made specificity can be selected and used in living cells to track, modulate or interfere with intracellular processes.
Specific GFP-binding artificial proteins (alphaRep): a new tool for in vitro to live cell applications.,Chevrel A, Urvoas A, de la Sierra-Gallay IL, Aumont-Nicaise M, Moutel S, Desmadril M, Perez F, Gautreau A, van Tilbeurgh H, Minard P, Valerio-Lepiniec M Biosci Rep. 2015 Jun 12;35(4). pii: e00223. doi: 10.1042/BSR20150080. PMID:26182430[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chevrel A, Urvoas A, de la Sierra-Gallay IL, Aumont-Nicaise M, Moutel S, Desmadril M, Perez F, Gautreau A, van Tilbeurgh H, Minard P, Valerio-Lepiniec M. Specific GFP-binding artificial proteins (alphaRep): a new tool for in vitro to live cell applications. Biosci Rep. 2015 Jun 12;35(4). pii: e00223. doi: 10.1042/BSR20150080. PMID:26182430 doi:http://dx.doi.org/10.1042/BSR20150080
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