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| | <StructureSection load='4xxf' size='340' side='right'caption='[[4xxf]], [[Resolution|resolution]] 1.34Å' scene=''> | | <StructureSection load='4xxf' size='340' side='right'caption='[[4xxf]], [[Resolution|resolution]] 1.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xxf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_yeast_cbs_8927 Antarctic yeast cbs 8927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XXF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glaciozyma_antarctica Glaciozyma antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XXF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xxf OCA], [https://pdbe.org/4xxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xxf RCSB], [https://www.ebi.ac.uk/pdbsum/4xxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xxf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xxf OCA], [http://pdbe.org/4xxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xxf RCSB], [http://www.ebi.ac.uk/pdbsum/4xxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xxf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0J9X279_9BASI A0A0J9X279_9BASI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Antarctic yeast cbs 8927]] | + | [[Category: Glaciozyma antarctica]] |
| - | [[Category: L-fuculose-phosphate aldolase]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bakar, F D.Abu]] | + | [[Category: Abdul Murad AM]] |
| - | [[Category: Beddoe, T]] | + | [[Category: Abu Bakar FD]] |
| - | [[Category: Illias, R]] | + | [[Category: Beddoe T]] |
| - | [[Category: Jaafar, N R]] | + | [[Category: Illias R]] |
| - | [[Category: Littler, D]] | + | [[Category: Jaafar NR]] |
| - | [[Category: Mahadi, M N]] | + | [[Category: Littler D]] |
| - | [[Category: Murad, A M.Abdul]]
| + | [[Category: Mahadi MN]] |
| - | [[Category: Rossjohn, J]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Fuculose 1-phosphate aldolase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Metalloenzyme]]
| + | |
| - | [[Category: Psychrophile]]
| + | |
| Structural highlights
Function
A0A0J9X279_9BASI
Publication Abstract from PubMed
Fuculose-1-phosphate aldolase (FucA) catalyses the reversible cleavage of L-fuculose 1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde. This enzyme from mesophiles and thermophiles has been extensively studied; however, there is no report on this enzyme from a psychrophile. In this study, the gene encoding FucA from Glaciozyma antarctica PI12 (GaFucA) was cloned and the enzyme was overexpressed in Escherichia coli, purified and crystallized. The tetrameric structure of GaFucA was determined to 1.34 A resolution. The overall architecture of GaFucA and its catalytically essential histidine triad are highly conserved among other fuculose aldolases. Comparisons of structural features between GaFucA and its mesophilic and thermophilic homologues revealed that the enzyme has typical psychrophilic attributes, indicated by the presence of a high number of nonpolar residues at the surface and a lower number of arginine residues.
Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12.,Jaafar NR, Littler D, Beddoe T, Rossjohn J, Illias RM, Mahadi NM, Mackeen MM, Murad AM, Abu Bakar FD Acta Crystallogr F Struct Biol Commun. 2016 Nov 1;72(Pt 11):831-839. doi:, 10.1107/S2053230X16015612. Epub 2016 Oct 27. PMID:27827354[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jaafar NR, Littler D, Beddoe T, Rossjohn J, Illias RM, Mahadi NM, Mackeen MM, Murad AM, Abu Bakar FD. Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12. Acta Crystallogr F Struct Biol Commun. 2016 Nov 1;72(Pt 11):831-839. doi:, 10.1107/S2053230X16015612. Epub 2016 Oct 27. PMID:27827354 doi:http://dx.doi.org/10.1107/S2053230X16015612
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