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| | ==The reduced form of OxyR regulatory domain from Psedomonas aeruginosa== | | ==The reduced form of OxyR regulatory domain from Psedomonas aeruginosa== |
| - | <StructureSection load='4y0m' size='340' side='right' caption='[[4y0m]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4y0m' size='340' side='right'caption='[[4y0m]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4y0m]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4y0m]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y0M FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4x6g|4x6g]], [[4xws|4xws]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0m OCA], [https://pdbe.org/4y0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y0m RCSB], [https://www.ebi.ac.uk/pdbsum/4y0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0m ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">oxyR, PA5344 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0m OCA], [http://pdbe.org/4y0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y0m RCSB], [http://www.ebi.ac.uk/pdbsum/4y0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0m ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HTL4_PSEAE Q9HTL4_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4y0m" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4y0m" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseae]] | + | [[Category: Large Structures]] |
| - | [[Category: Ha, N C]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Jo, I]] | + | [[Category: Ha NC]] |
| - | [[Category: Kim, J S]] | + | [[Category: Jo I]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Kim JS]] |
| - | [[Category: Lysr]]
| + | |
| - | [[Category: Oxyr]]
| + | |
| - | [[Category: Peroxide]]
| + | |
| - | [[Category: Transcription regulator]]
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| Structural highlights
Function
Q9HTL4_PSEAE
Publication Abstract from PubMed
OxyR, a bacterial peroxide sensor, is a LysR-type transcriptional regulator (LTTR) that regulates the transcription of defense genes in response to a low level of cellular H2O2. Consisting of an N-terminal DNA-binding domain (DBD) and a C-terminal regulatory domain (RD), OxyR senses H2O2 with conserved cysteine residues in the RD. However, the precise mechanism of OxyR is not yet known due to the absence of the full-length (FL) protein structure. Here we determined the crystal structures of the FL protein and RD of Pseudomonas aeruginosa OxyR and its C199D mutant proteins. The FL crystal structures revealed that OxyR has a tetrameric arrangement assembled via two distinct dimerization interfaces. The C199D mutant structures suggested that new interactions that are mediated by cysteine hydroxylation induce a large conformational change, facilitating intramolecular disulfide-bond formation. More importantly, a bound H2O2 molecule was found near the Cys199 site, suggesting the H2O2-driven oxidation mechanism of OxyR. Combined with the crystal structures, a modeling study suggested that a large movement of the DBD is triggered by structural changes in the regulatory domains upon oxidation. Taken together, these findings provide novel concepts for answering key questions regarding OxyR in the H2O2-sensing and oxidation-dependent regulation of antioxidant genes.
Structural details of the OxyR peroxide-sensing mechanism.,Jo I, Chung IY, Bae HW, Kim JS, Song S, Cho YH, Ha NC Proc Natl Acad Sci U S A. 2015 May 19;112(20):6443-8. doi:, 10.1073/pnas.1424495112. Epub 2015 Apr 30. PMID:25931525[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jo I, Chung IY, Bae HW, Kim JS, Song S, Cho YH, Ha NC. Structural details of the OxyR peroxide-sensing mechanism. Proc Natl Acad Sci U S A. 2015 May 19;112(20):6443-8. doi:, 10.1073/pnas.1424495112. Epub 2015 Apr 30. PMID:25931525 doi:http://dx.doi.org/10.1073/pnas.1424495112
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