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| | <StructureSection load='4y0w' size='340' side='right'caption='[[4y0w]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='4y0w' size='340' side='right'caption='[[4y0w]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4y0w]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao579 Pseudomonas aeruginosa pao579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4y0w]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO579 Pseudomonas aeruginosa PAO579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y0W FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A161_18155 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1191475 Pseudomonas aeruginosa PAO579])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0w OCA], [https://pdbe.org/4y0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y0w RCSB], [https://www.ebi.ac.uk/pdbsum/4y0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0w ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0w OCA], [http://pdbe.org/4y0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y0w RCSB], [http://www.ebi.ac.uk/pdbsum/4y0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A140UHA7_PSEAI A0A140UHA7_PSEAI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseudomonas aeruginosa pao579]] | + | [[Category: Pseudomonas aeruginosa PAO579]] |
| - | [[Category: Milani, M]] | + | [[Category: Milani M]] |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Essential protein involved in the biosynthesis of threonylcarbamoyl adenosine]]
| + | |
| Structural highlights
Function
A0A140UHA7_PSEAI
Publication Abstract from PubMed
The Pseudomonas aeruginosa PA3685 locus encodes a conserved protein that shares 49% sequence identity with Escherichiacoli YeaZ, which was recently reported as involved in the biosynthesis of threonylcarbamoyl adenosine (t6A), a universal modified tRNA nucleoside. Many YeaZ orthologues were reported as "essential for life" among various bacterial species, suggesting a critical role for both these proteins and for the t6A biosynthetic pathway. We provide here evidences that PA3685 protein (PaYeaZ) is essential. Additionally, we describe its purification, crystallization, and crystallographic structure. The crystal structure shows that PaYeaZ is composed of two domains one of which is the platform to form protein-protein interaction involved either in homodimeric assembly or in the formation of the multiprotein complex required for the synthesis of t6A. These features make the PaYeaZ protein a potential target candidate for the design of novel inhibitors able to hinder the complex formation and expected to abolish the crucial activity of t6A synthesis.
Crystal structure of YeaZ from Pseudomonas aeruginosa.,Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G Biochem Biophys Res Commun. 2016 Jan 6. pii: S0006-291X(16)30008-0. doi:, 10.1016/j.bbrc.2016.01.008. PMID:26768361[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G. Crystal structure of YeaZ from Pseudomonas aeruginosa. Biochem Biophys Res Commun. 2016 Jan 6. pii: S0006-291X(16)30008-0. doi:, 10.1016/j.bbrc.2016.01.008. PMID:26768361 doi:http://dx.doi.org/10.1016/j.bbrc.2016.01.008
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