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| | <StructureSection load='4y1p' size='340' side='right'caption='[[4y1p]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='4y1p' size='340' side='right'caption='[[4y1p]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4y1p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulac Sulac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4y1p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y1P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPM:3-ISOPROPYLMALIC+ACID'>IPM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPM:3-ISOPROPYLMALIC+ACID'>IPM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">leuB, Saci_0600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330779 SULAC])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1p OCA], [https://pdbe.org/4y1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y1p RCSB], [https://www.ebi.ac.uk/pdbsum/4y1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1p ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1p OCA], [http://pdbe.org/4y1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y1p RCSB], [http://www.ebi.ac.uk/pdbsum/4y1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q4JB37_SULAC Q4JB37_SULAC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 3-isopropylmalate dehydrogenase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sulac]] | + | [[Category: Sulfolobus acidocaldarius DSM 639]] |
| - | [[Category: Kuzuyama, T]] | + | [[Category: Kuzuyama T]] |
| - | [[Category: Nishiyama, M]] | + | [[Category: Nishiyama M]] |
| - | [[Category: Takahashi, K]] | + | [[Category: Takahashi K]] |
| - | [[Category: Tomita, T]] | + | [[Category: Tomita T]] |
| - | [[Category: Beta-decarboxylating dehyrogenase]]
| + | |
| - | [[Category: Closed form]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Sulfolobus acidocaldarius]]
| + | |
| Structural highlights
Function
Q4JB37_SULAC
Publication Abstract from PubMed
Sulfolobus acidocaldarius, a hyperthermoacidophilic archaeon, possesses two beta-decarboxylating dehydrogenase genes, saci_0600 and saci_2375, in its genome, which suggests that it uses these enzymes for three similar reactions in lysine biosynthesis through 2-aminoadipate, leucine biosynthesis, and the tricarboxylic acid cycle. To elucidate their roles, these two genes were expressed in Escherichia coli in the present study and their gene products were characterized. Saci_0600 recognized 3-isopropylmalate as a substrate, but exhibited slight and no activity for homoisocitrate and isocitrate, respectively. Saci_2375 exhibited distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. These results suggest that Saci_0600 is a 3-isopropylmalate dehydrogenase for leucine biosynthesis and Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase. The crystal structure of Saci_0600 was determined as a closed-form complex that binds 3-isopropylmalate and Mg2+, thereby revealing the structural basis for the extreme thermostability and novel-type recognition of the 3-isopropyl moiety of the substrate.
Characterization of two beta-decarboxylating dehydrogenases from Sulfolobus acidocaldarius.,Takahashi K, Nakanishi F, Tomita T, Akiyama N, Lassak K, Albers SV, Kuzuyama T, Nishiyama M Extremophiles. 2016 Nov;20(6):843-853. Epub 2016 Sep 2. PMID:27590116[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Takahashi K, Nakanishi F, Tomita T, Akiyama N, Lassak K, Albers SV, Kuzuyama T, Nishiyama M. Characterization of two beta-decarboxylating dehydrogenases from Sulfolobus acidocaldarius. Extremophiles. 2016 Nov;20(6):843-853. Epub 2016 Sep 2. PMID:27590116 doi:http://dx.doi.org/10.1007/s00792-016-0872-4
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