This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4y4y

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 18:06, 26 April 2023

T=1 capsid structure of SeMV Ndel65CP fused with B-domain of S. aureus protein SpA at the N-terminus (C2 crystal form)

Structural highlights

4y4y is a 30 chain structure with sequence from Sesbania mosaic virus and Staphylococcus aureus subsp. aureus NCTC 8325. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPA_STAA8 Q9EB06_9VIRU

Publication Abstract from PubMed

The capsid protein (CP) of Sesbania mosaic virus (SeMV, a T=3 plant virus) consists of a disordered N-terminal R-domain and an ordered S-domain. Removal of the R-domain results in the formation of T=1 particles. In the current study, the R-domain was replaced with unrelated polypeptides of similar lengths: the B-domain of Staphylococcus aureus SpA, and SeMV encoded polypeptides P8 and P10. The chimeric proteins contained T=3 or larger virus-like particles (VLPs) and could not be crystallized. The presence of metal ions during purification resulted in a large number of heterogeneous nucleoprotein complexes. N65-B (R domain replaced with B domain) could also be purified in a dimeric form. Its crystal structure revealed T=1 particles devoid of metal ions and the B-domain was disordered. However, the B-domain was functional in N65-B VLPs, suggesting possible biotechnological applications. These studies illustrate the importance of N-terminal residues, metal ions and robustness of the assembly process.

Structural studies on chimeric Sesbania mosaic virus coat protein: Revisiting SeMV assembly.,Gulati A, Murthy A, Abraham A, Mohan K, Natraj U, Savithri HS, Murthy MR Virology. 2015 Dec 17;489:34-43. doi: 10.1016/j.virol.2015.11.029. PMID:26704627^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gulati A, Murthy A, Abraham A, Mohan K, Natraj U, Savithri HS, Murthy MR. Structural studies on chimeric Sesbania mosaic virus coat protein: Revisiting SeMV assembly. Virology. 2015 Dec 17;489:34-43. doi: 10.1016/j.virol.2015.11.029. PMID:26704627 doi:http://dx.doi.org/10.1016/j.virol.2015.11.029

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4y4y"

Categories: Large Structures | Sesbania mosaic virus | Staphylococcus aureus subsp. aureus NCTC 8325 | Gulati A | Murthy MRN

@@ Line 3: / Line 3: @@
 <StructureSection load='4y4y' size='340' side='right'caption='[[4y4y]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4y4y]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Sesbania_mosaic_virus Sesbania mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y4Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y4Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4y4y]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Sesbania_mosaic_virus Sesbania mosaic virus] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y4Y FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1vak|1vak]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y4y OCA], [https://pdbe.org/4y4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y4y RCSB], [https://www.ebi.ac.uk/pdbsum/4y4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y4y ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spa, SAOUHSC_00069 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12558 Sesbania mosaic virus])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y4y OCA], [http://pdbe.org/4y4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y4y RCSB], [http://www.ebi.ac.uk/pdbsum/4y4y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y4y ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPA_STAA8 SPA_STAA8] [https://www.uniprot.org/uniprot/Q9EB06_9VIRU Q9EB06_9VIRU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 24: @@
 [[Category: Large Structures]]
 [[Category: Sesbania mosaic virus]]
-[[Category: Gulati, A]]
+[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-[[Category: Murthy, M R.N]]
+[[Category: Gulati A]]
-[[Category: Chimeric vlp]]
+[[Category: Murthy MRN]]
-[[Category: Coat protein]]
-[[Category: In vitro assembly]]
-[[Category: Virus]]

4y4y

From Proteopedia

Revision as of 18:06, 26 April 2023

T=1 capsid structure of SeMV Ndel65CP fused with B-domain of S. aureus protein SpA at the N-terminus (C2 crystal form)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox