8b4h
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==IstA transposase cleaved donor complex== | |
| + | <StructureSection load='8b4h' size='340' side='right'caption='[[8b4h]], [[Resolution|resolution]] 3.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8b4h]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B4H FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b4h OCA], [https://pdbe.org/8b4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b4h RCSB], [https://www.ebi.ac.uk/pdbsum/8b4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b4h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TRA6_GEOSE TRA6_GEOSE] Involved in the transposition of the insertion sequence. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad impacts on gene expression, genome evolution, and the spread of drug-resistance in bacteria. Here, we use biochemical and structural approaches to define the molecular determinants by which IstA, a transposase present in the widespread IS21 family of mobile elements, catalyzes efficient DNA transposition. Solution studies show that IstA engages the transposon terminal sequences to form a high-molecular weight complex and promote DNA integration. A 3.4 A resolution structure of the transposase bound to transposon ends corroborates our biochemical findings and reveals that IstA self-assembles into a highly intertwined tetramer that synapses two supercoiled terminal inverted repeats. The three-dimensional organization of the IstA*DNA cleaved donor complex reveals remarkable similarities with retroviral integrases and classic transposase systems, such as Tn7 and bacteriophage Mu, and provides insights into IS21 transposition. | ||
| - | + | IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings.,Spinola-Amilibia M, Araujo-Bazan L, de la Gandara A, Berger JM, Arias-Palomo E Nat Commun. 2023 Apr 22;14(1):2335. doi: 10.1038/s41467-023-38071-x. PMID:37087515<ref>PMID:37087515</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8b4h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geobacillus stearothermophilus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Araujo-Bazan L]] | ||
| + | [[Category: Arias-Palomo E]] | ||
| + | [[Category: Berger JM]] | ||
| + | [[Category: Spinola-Amilibia M]] | ||
| + | [[Category: De la Gandara A]] | ||
Revision as of 07:28, 3 May 2023
IstA transposase cleaved donor complex
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