|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4ydz' size='340' side='right'caption='[[4ydz]], [[Resolution|resolution]] 3.60Å' scene=''> | | <StructureSection load='4ydz' size='340' side='right'caption='[[4ydz]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ydz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YDZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ydz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YDZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w1z|3w1z]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ydz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ydz OCA], [https://pdbe.org/4ydz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ydz RCSB], [https://www.ebi.ac.uk/pdbsum/4ydz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ydz ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sip-1, F43D9.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ydz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ydz OCA], [http://pdbe.org/4ydz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ydz RCSB], [http://www.ebi.ac.uk/pdbsum/4ydz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ydz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SIP1_CAEEL SIP1_CAEEL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Caeel]] | + | [[Category: Caenorhabditis elegans]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Buchner, J]] | + | [[Category: Buchner J]] |
| - | [[Category: Daake, M]] | + | [[Category: Daake M]] |
| - | [[Category: Fleckenstein, T]] | + | [[Category: Fleckenstein T]] |
| - | [[Category: Groll, M]] | + | [[Category: Groll M]] |
| - | [[Category: Haslbeck, M]] | + | [[Category: Haslbeck M]] |
| - | [[Category: Kastenmueller, A]] | + | [[Category: Kastenmueller A]] |
| - | [[Category: Krause, M]] | + | [[Category: Krause M]] |
| - | [[Category: Peters, C]] | + | [[Category: Peters C]] |
| - | [[Category: Stein, M L]] | + | [[Category: Stein ML]] |
| - | [[Category: Weinfurtner, D]] | + | [[Category: Weinfurtner D]] |
| - | [[Category: Weinkauf, S]] | + | [[Category: Weinkauf S]] |
| - | [[Category: Chaperone]]
| + | |
| - | [[Category: Heat shock]]
| + | |
| - | [[Category: Molecular chaperone]]
| + | |
| - | [[Category: Protein aggregation]]
| + | |
| - | [[Category: Shsp]]
| + | |
| Structural highlights
Function
SIP1_CAEEL
Publication Abstract from PubMed
Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of reproducing adults and embryos. X-ray crystallography and electron microscopy revealed that Sip1 exists in a range of well-defined globular assemblies consisting of two half-spheres, each made of dimeric "spokes." Strikingly, the oligomeric distribution of Sip1 as well as its chaperone activity depend on pH, with a trend toward smaller species and higher activity at acidic conditions such as present in nematode eggs. The analysis of the interactome shows that Sip1 has a specific substrate spectrum including proteins that are essential for embryo development.
The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes.,Fleckenstein T, Kastenmuller A, Stein ML, Peters C, Daake M, Krause M, Weinfurtner D, Haslbeck M, Weinkauf S, Groll M, Buchner J Mol Cell. 2015 May 22. pii: S1097-2765(15)00301-9. doi:, 10.1016/j.molcel.2015.04.019. PMID:26009280[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fleckenstein T, Kastenmuller A, Stein ML, Peters C, Daake M, Krause M, Weinfurtner D, Haslbeck M, Weinkauf S, Groll M, Buchner J. The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes. Mol Cell. 2015 May 22. pii: S1097-2765(15)00301-9. doi:, 10.1016/j.molcel.2015.04.019. PMID:26009280 doi:http://dx.doi.org/10.1016/j.molcel.2015.04.019
|
