1k0x

From Proteopedia

Revision as of 15:39, 12 November 2007

Solution Structure of Melanoma Inhibitory Activity Protein

Overview

Melanoma inhibitory activity (MIA) is a small secreted protein that is, implicated in cartilage cell maintenance and melanoma metastasis. It is, representative of a recently discovered family of proteins that contain a, Src Homologous 3 (SH3) subdomain. While SH3 domains are normally found in, intracellular proteins and mediate protein-protein interactions via, recognition of polyproline helices, MIA is single-domain extracellular, protein, and it probably binds to a different class of ligands. Here we, report the assignments, solution structure, and dynamics of human MIA, determined by heteronuclear NMR methods. The structures were calculated in, a semi-automated manner without manual assignment of NOE crosspeaks, and, have a backbone rmsd of 0.38 A over the ordered regions of the protein., The structure consists of an SH3-like subdomain with N- and C-terminal, extensions of approximately 20 amino acids each that together form a novel, fold. The rmsd between the solution structure and our recently reported, crystal structure is 0.86 A over the ordered regions of the backbone, and, the main differences are localized to the most dynamic regions of the, protein. The similarity between the NMR and crystal structures supports, the use of automated NOE assignments and ambiguous restraints to, accelerate the calculation of NMR structures.

Disease

Known disease associated with this structure: Myocardial infarction, susceptibility to OMIM:[611082]

About this Structure

1K0X is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Solution structure and dynamics of melanoma inhibitory activity protein., Lougheed JC, Domaille PJ, Handel TM, J Biomol NMR. 2002 Mar;22(3):211-23. PMID:11991352

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