8bx8
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==In situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state== | |
| + | <StructureSection load='8bx8' size='340' side='right'caption='[[8bx8]], [[Resolution|resolution]] 30.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8bx8]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BX8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bx8 OCA], [https://pdbe.org/8bx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bx8 RCSB], [https://www.ebi.ac.uk/pdbsum/8bx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bx8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/I7M008_TETTS I7M008_TETTS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Axonemal outer dynein arm (ODA) motors generate force for ciliary beating. We analyzed three states of the ODA during the power stroke cycle using in situ cryo-electron tomography, subtomogram averaging, and classification. These states of force generation depict the prepower stroke, postpower stroke, and intermediate state conformations. Comparison of these conformations to published in vitro atomic structures of cytoplasmic dynein, ODA, and the Shulin-ODA complex revealed differences in the orientation and position of the dynein head. Our analysis shows that in the absence of ATP, all dynein linkers interact with the AAA3/AAA4 domains, indicating that interactions with the adjacent microtubule doublet B-tubule direct dynein orientation. For the prepower stroke conformation, there were changes in the tail that is anchored on the A-tubule. We built models starting with available high-resolution structures to generate a best-fitting model structure for the in situ pre- and postpower stroke ODA conformations, thereby showing that ODA in a complex with Shulin adopts a similar conformation as the active prepower stroke ODA in the axoneme. | ||
| - | + | ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography.,Zimmermann N, Noga A, Obbineni JM, Ishikawa T EMBO J. 2023 Apr 13:e112466. doi: 10.15252/embj.2022112466. PMID:37051721<ref>PMID:37051721</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8bx8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Tetrahymena thermophila]] | ||
| + | [[Category: Ishikawa T]] | ||
| + | [[Category: Noga A]] | ||
| + | [[Category: Obbineni JM]] | ||
| + | [[Category: Zimmermann NEL]] | ||
Revision as of 06:52, 10 May 2023
In situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state
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