7bvi
From Proteopedia
(Difference between revisions)
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==Crystal structure of Pennisetum glaucum monodehydroascorbate reductase== | ==Crystal structure of Pennisetum glaucum monodehydroascorbate reductase== | ||
| - | <StructureSection load='7bvi' size='340' side='right'caption='[[7bvi]]' scene=''> | + | <StructureSection load='7bvi' size='340' side='right'caption='[[7bvi]], [[Resolution|resolution]] 2.39Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BVI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bvi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cenchrus_americanus Cenchrus americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BVI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvi OCA], [https://pdbe.org/7bvi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bvi RCSB], [https://www.ebi.ac.uk/pdbsum/7bvi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvi ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvi OCA], [https://pdbe.org/7bvi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bvi RCSB], [https://www.ebi.ac.uk/pdbsum/7bvi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ascorbate (AsA) is a crucial antioxidant in plants, and its recycling is necessary for protecting cells from oxidative damage and imparting stress tolerance. The monodehydroascorbate reductase (MDHAR) enzyme of the ascorbate-glutathione pathway plays a vital role in recycling AsA from monodehydroascorbate (MDHA) radical. Pennisetum glaucum (Pg), also known as pearl millet, is known to be more tolerant to abiotic stress than other food crops, such as rice. However, the contribution of MDHAR from this sessile plant to its unique stress tolerance mechanism is not well understood. In this study, we isolated a gene encoding the MDHAR enzyme from heat stress-adapted pearl millet and characterized it using enzyme kinetics, thermal stability assays, and crystal structure determination. Our results indicate that PgMDHAR is a more robust enzyme than its rice counterpart (Oryza sativa; Os). We solved the crystal structure of PgMDHAR at 1.8 A and found that the enzyme has a more compact structure and greater stability than OsMDHAR. Using hybrid quantum mechanics and molecular mechanics calculations, we demonstrate that the structure of PgMDHAR contributes to increased stability towards bound FAD. Overall, the higher structural stability and affinity for NADH demonstrated by PgMDHAR are expected to impart improved stress tolerance. Our findings suggest that transgenic food crops expressing MDHAR from stress-adapted pearl millet may exhibit better tolerance to oxidative stress in the unpredictable climatic conditions prevalent today. | ||
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| + | Biochemical and structural characterization of a robust and thermostable ascorbate recycling monodehydroascorbate reductase (MDHAR) from stress adapted pearl millet.,Sonkar KS, Achary VMM, Sahoo S, Reddy MK, Arockiasamy A Biochem Biophys Res Commun. 2023 Jun 25;662:135-141. doi: , 10.1016/j.bbrc.2023.04.034. Epub 2023 Apr 19. PMID:37119729<ref>PMID:37119729</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bvi" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Cenchrus americanus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Achary MM]] | [[Category: Achary MM]] | ||
Revision as of 07:01, 10 May 2023
Crystal structure of Pennisetum glaucum monodehydroascorbate reductase
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