1k2a
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(New page: 200px<br /> <applet load="1k2a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k2a, resolution 1.00Å" /> '''Modified Form of Eo...)
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Revision as of 15:40, 12 November 2007
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Modified Form of Eosinophil-derived Neurotoxin
Contents |
Overview
The crystal structure of a post-translationally modified form of, eosinophil-derived neurotoxin (EDN) with four extra residues on its N, terminus ((-4)EDN) has been solved and refined at atomic resolution (1 A)., Two of the extra residues can be placed unambiguously, while the density, corresponding to two others is poor. The modified N terminus appears to, influence the position of the catalytically important His129, possibly, explaining the diminished catalytic activity of this variant. However, (-4)EDN has been shown to be cytotoxic to a Kaposi's sarcoma tumor cell, line and other endothelial cell lines. Analysis of the structure and, function suggests that the reason for cytotoxicity is most likely due to, cellular recognition by the N-terminal extension, since the intrinsic, activity of the enzyme is not sufficient for cytotoxicity and the, N-terminal extension does not affect the conformation of EDN.
Disease
Known diseases associated with this structure: Central hypoventilation syndrome, congenital OMIM:[131242], Hirschsprung disease OMIM:[131242], Shah-Waardenburg syndrome OMIM:[131242]
About this Structure
1K2A is a Single protein structure of sequence from Homo sapiens with SO4 as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities., Chang C, Newton DL, Rybak SM, Wlodawer A, J Mol Biol. 2002 Mar 15;317(1):119-30. PMID:11916383
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