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| | <StructureSection load='4yuz' size='340' side='right'caption='[[4yuz]], [[Resolution|resolution]] 1.97Å' scene=''> | | <StructureSection load='4yuz' size='340' side='right'caption='[[4yuz]], [[Resolution|resolution]] 1.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4yuz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycc Trycc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YUZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4yuz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_strain_CL_Brener Trypanosoma cruzi strain CL Brener]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YUZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4JV:5-[(4-METHYLBENZYL)OXY]QUINAZOLINE-2,4-DIAMINE'>4JV</scene>, <scene name='pdbligand=S4M:5-[(S)-(3-AMINOPROPYL)(METHYL)-LAMBDA~4~-SULFANYL]-5-DEOXYADENOSINE'>S4M</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4JV:5-[(4-METHYLBENZYL)OXY]QUINAZOLINE-2,4-DIAMINE'>4JV</scene>, <scene name='pdbligand=S4M:5-[(S)-(3-AMINOPROPYL)(METHYL)-LAMBDA~4~-SULFANYL]-5-DEOXYADENOSINE'>S4M</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yuv|4yuv]], [[4yuw|4yuw]], [[4yux|4yux]], [[4yuy|4yuy]], [[4yv0|4yv0]], [[4yv1|4yv1]], [[4yv2|4yv2]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuz OCA], [https://pdbe.org/4yuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yuz RCSB], [https://www.ebi.ac.uk/pdbsum/4yuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yuz ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tc00.1047053510339.50 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=353153 TRYCC])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuz OCA], [http://pdbe.org/4yuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yuz RCSB], [http://www.ebi.ac.uk/pdbsum/4yuz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yuz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q4DA73_TRYCC Q4DA73_TRYCC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Spermidine Synthase|Spermidine Synthase]] | + | *[[Spermidine synthase 3D structures|Spermidine synthase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Spermidine synthase]] | + | [[Category: Trypanosoma cruzi strain CL Brener]] |
| - | [[Category: Trycc]]
| + | [[Category: Amano Y]] |
| - | [[Category: Amano, Y]] | + | [[Category: Tateishi Y]] |
| - | [[Category: Tateishi, Y]] | + | |
| - | [[Category: Methyltransferase]]
| + | |
| - | [[Category: Polyamine synthesis]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q4DA73_TRYCC
Publication Abstract from PubMed
Trypanosoma cruzi causes Chagas disease, a severe disease affecting 8-10 million people in Latin America. While nifurtimox and benznidazole are used to treat this disease, their efficacy is limited and adverse effects are observed. New therapeutic targets and novel drugs are therefore urgently required. Enzymes in the polyamine-trypanothione pathway are promising targets for the treatment of Chagas disease. Spermidine synthase is a key enzyme in this pathway that catalyzes the transfer of an aminopropyl group from decarboxylated S-adenosylmethionine (dcSAM) to putrescine. Fragment-based drug discovery was therefore conducted to identify novel, potent inhibitors of spermidine synthase from T. cruzi (TcSpdSyn). Here, crystal structures of TcSpdSyn in complex with dcSAM, trans-4-methylcyclohexylamine and hit compounds from fragment screening are reported. The structure of dcSAM complexed with TcSpdSyn indicates that dcSAM stabilizes the conformation of the `gatekeeping' loop to form the putrescine-binding pocket. The structures of fragments bound to TcSpdSyn revealed two fragment-binding sites: the putrescine-binding pocket and the dimer interface. The putrescine-binding pocket was extended by an induced-fit mechanism. The crystal structures indicate that the conformation of the dimer interface is required to stabilize the gatekeeping loop and that fragments binding to this interface inhibit TcSpdSyn by disrupting its conformation. These results suggest that utilizing the dynamic structural changes in TcSpdSyn that occur upon inhibitor binding will facilitate the development of more selective and potent inhibitors.
Structural insights into the novel inhibition mechanism of Trypanosoma cruzi spermidine synthase.,Amano Y, Namatame I, Tateishi Y, Honboh K, Tanabe E, Niimi T, Sakashita H Acta Crystallogr D Biol Crystallogr. 2015 Sep;71(Pt 9):1879-89. doi:, 10.1107/S1399004715013048. Epub 2015 Aug 25. PMID:26327378[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Amano Y, Namatame I, Tateishi Y, Honboh K, Tanabe E, Niimi T, Sakashita H. Structural insights into the novel inhibition mechanism of Trypanosoma cruzi spermidine synthase. Acta Crystallogr D Biol Crystallogr. 2015 Sep;71(Pt 9):1879-89. doi:, 10.1107/S1399004715013048. Epub 2015 Aug 25. PMID:26327378 doi:http://dx.doi.org/10.1107/S1399004715013048
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