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4yv4

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Revision as of 07:27, 10 May 2023

Structure of the C. elegans SAS-5 coiled coil domain

Structural highlights

4yv4 is a 8 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAS5_CAEEL Required for centrosome duplication. Essential for daughter-centriole formation. Requires both maternal and partenal expression, suggesting that it regulates centriole duplication during both spermatogenesis and early embryogenesis.^[1] ^[2]

Publication Abstract from PubMed

Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In C. elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional homologs across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo.

The protein SAS-5 forms large oligomeric assemblies critical for centriole formation.,Rogala KB, Dynes NJ, Hatzopoulos GN, Yan J, Pong SK, Robinson CV, Deane CM, Gonczy P, Vakonakis I Elife. 2015 May 29;4. doi: 10.7554/eLife.07410. PMID:26023830^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Delattre M, Leidel S, Wani K, Baumer K, Bamat J, Schnabel H, Feichtinger R, Schnabel R, Gonczy P. Centriolar SAS-5 is required for centrosome duplication in C. elegans. Nat Cell Biol. 2004 Jul;6(7):656-64. PMID:15232593 doi:http://dx.doi.org/10.1038/ncb1146
↑ Dammermann A, Muller-Reichert T, Pelletier L, Habermann B, Desai A, Oegema K. Centriole assembly requires both centriolar and pericentriolar material proteins. Dev Cell. 2004 Dec;7(6):815-29. PMID:15572125 doi:S1534580704003806
↑ Rogala KB, Dynes NJ, Hatzopoulos GN, Yan J, Pong SK, Robinson CV, Deane CM, Gonczy P, Vakonakis I. The protein SAS-5 forms large oligomeric assemblies critical for centriole formation. Elife. 2015 May 29;4. doi: 10.7554/eLife.07410. PMID:26023830 doi:http://dx.doi.org/10.7554/eLife.07410

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4yv4"

Categories: Caenorhabditis elegans | Large Structures | Hatzopoulos GN | Rogala KB | Vakonakis I

@@ Line 3: / Line 3: @@
 <StructureSection load='4yv4' size='340' side='right'caption='[[4yv4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4yv4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YV4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YV4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4yv4]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YV4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ynh|4ynh]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yv4 OCA], [https://pdbe.org/4yv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yv4 RCSB], [https://www.ebi.ac.uk/pdbsum/4yv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yv4 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sas-5, F35B12.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yv4 OCA], [http://pdbe.org/4yv4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yv4 RCSB], [http://www.ebi.ac.uk/pdbsum/4yv4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yv4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SAS5_CAEEL SAS5_CAEEL]] Required for centrosome duplication. Essential for daughter-centriole formation. Requires both maternal and partenal expression, suggesting that it regulates centriole duplication during both spermatogenesis and early embryogenesis.<ref>PMID:15232593</ref> <ref>PMID:15572125</ref>
+[https://www.uniprot.org/uniprot/SAS5_CAEEL SAS5_CAEEL] Required for centrosome duplication. Essential for daughter-centriole formation. Requires both maternal and partenal expression, suggesting that it regulates centriole duplication during both spermatogenesis and early embryogenesis.<ref>PMID:15232593</ref> <ref>PMID:15572125</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Hatzopoulos, G N]]
+[[Category: Hatzopoulos GN]]
-[[Category: Rogala, K B]]
+[[Category: Rogala KB]]
-[[Category: Vakonakis, I]]
+[[Category: Vakonakis I]]
-[[Category: A-helical]]
-[[Category: Cell cycle]]
-[[Category: Coiled coil]]
-[[Category: Structural protein]]
-[[Category: Trimer]]

4yv4

From Proteopedia

Revision as of 07:27, 10 May 2023

Structure of the C. elegans SAS-5 coiled coil domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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