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| | <StructureSection load='4ywf' size='340' side='right'caption='[[4ywf]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4ywf' size='340' side='right'caption='[[4ywf]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ywf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"verrucosispora_maris"_fiedler_et_al._2008 "verrucosispora maris" fiedler et al. 2008]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YWF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ywf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_maris Micromonospora maris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YWF FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abyA5, VAB18032_16440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1003110 "Verrucosispora maris" Fiedler et al. 2008])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ywf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ywf OCA], [https://pdbe.org/4ywf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ywf RCSB], [https://www.ebi.ac.uk/pdbsum/4ywf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ywf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ywf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ywf OCA], [http://pdbe.org/4ywf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ywf RCSB], [http://www.ebi.ac.uk/pdbsum/4ywf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ywf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F4F7F5_MICM1 F4F7F5_MICM1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Verrucosispora maris fiedler et al. 2008]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Byrne, M J]] | + | [[Category: Micromonospora maris]] |
| - | [[Category: Race, P R]] | + | [[Category: Byrne MJ]] |
| - | [[Category: Deacetylase]] | + | [[Category: Race PR]] |
| - | [[Category: Elimination]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Spirotetronate]]
| + | |
| Structural highlights
Function
F4F7F5_MICM1
Publication Abstract from PubMed
Spirotetronate and spirotetramate natural products include a multitude of compounds with potent antimicrobial and antitumor activities. Their biosynthesis incorporates many unusual biocatalytic steps, including regio- and stereo-specific modifications, cyclizations promoted by Diels-Alderases, and acetylation-elimination reactions. Here we focus on the acetate elimination catalyzed by AbyA5, implicated in the formation of the key Diels-Alder substrate to give the spirocyclic system of the antibiotic abyssomicin C. Using synthetic substrate analogues, it is shown that AbyA5 catalyzes stereospecific acetate elimination, establishing the (R)-tetronate acetate as a biosynthetic intermediate. The X-ray crystal structure of AbyA5, the first of an acetate-eliminating enzyme, reveals a deviant acetyl esterase fold. Molecular dynamics simulations and enzyme assays show the use of a His-Ser dyad to catalyze either elimination or hydrolysis, via disparate mechanisms, under substrate control.
An Esterase-like Lyase Catalyzes Acetate Elimination in Spirotetronate/Spirotetramate Biosynthesis.,Lees NR, Han LC, Byrne MJ, Davies JA, Parnell AE, Moreland PEJ, Stach JEM, van der Kamp MW, Willis CL, Race PR Angew Chem Int Ed Engl. 2019 Feb 18;58(8):2305-2309. doi: 10.1002/anie.201812105., Epub 2019 Jan 21. PMID:30664319[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lees NR, Han LC, Byrne MJ, Davies JA, Parnell AE, Moreland PEJ, Stach JEM, van der Kamp MW, Willis CL, Race PR. An Esterase-like Lyase Catalyzes Acetate Elimination in Spirotetronate/Spirotetramate Biosynthesis. Angew Chem Int Ed Engl. 2019 Feb 18;58(8):2305-2309. doi: 10.1002/anie.201812105., Epub 2019 Jan 21. PMID:30664319 doi:http://dx.doi.org/10.1002/anie.201812105
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