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| | ==Complex of SpaO(SPOA1,2) and OrgB(APAR)::T4lysozyme fusion protein== | | ==Complex of SpaO(SPOA1,2) and OrgB(APAR)::T4lysozyme fusion protein== |
| - | <StructureSection load='4yx7' size='340' side='right' caption='[[4yx7]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4yx7' size='340' side='right'caption='[[4yx7]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4yx7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YX7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4yx7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YX7 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spaO, STM2891 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY]), orgB, STM2869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yx7 OCA], [https://pdbe.org/4yx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yx7 RCSB], [https://www.ebi.ac.uk/pdbsum/4yx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yx7 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yx7 OCA], [http://pdbe.org/4yx7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yx7 RCSB], [http://www.ebi.ac.uk/pdbsum/4yx7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yx7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPAO_SALTY SPAO_SALTY]] Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells. [[http://www.uniprot.org/uniprot/ORGB_SALTY ORGB_SALTY]] Oxygen-regulated protein required for bacterial internalization. | + | [https://www.uniprot.org/uniprot/SPAO_SALTY SPAO_SALTY] Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpt4]] | + | [[Category: Escherichia virus T4]] |
| - | [[Category: Lysozyme]] | + | [[Category: Large Structures]] |
| - | [[Category: Salty]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] |
| - | [[Category: Notti, R Q]] | + | [[Category: Notti RQ]] |
| - | [[Category: Stebbins, C E]] | + | [[Category: Stebbins CE]] |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Type iii secretion system]]
| + | |
| Structural highlights
Function
SPAO_SALTY Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells.
Publication Abstract from PubMed
Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.
A common assembly module in injectisome and flagellar type III secretion sorting platforms.,Notti RQ, Bhattacharya S, Lilic M, Stebbins CE Nat Commun. 2015 May 21;6:7125. doi: 10.1038/ncomms8125. PMID:25994170[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Notti RQ, Bhattacharya S, Lilic M, Stebbins CE. A common assembly module in injectisome and flagellar type III secretion sorting platforms. Nat Commun. 2015 May 21;6:7125. doi: 10.1038/ncomms8125. PMID:25994170 doi:http://dx.doi.org/10.1038/ncomms8125
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