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| | <StructureSection load='4z2f' size='340' side='right'caption='[[4z2f]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='4z2f' size='340' side='right'caption='[[4z2f]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z2f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Athelia_rolfsii Athelia rolfsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z2F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z2F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z2f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Athelia_rolfsii Athelia rolfsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z2F FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yld|4yld]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2f OCA], [https://pdbe.org/4z2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z2f RCSB], [https://www.ebi.ac.uk/pdbsum/4z2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z2f ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2f OCA], [http://pdbe.org/4z2f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z2f RCSB], [http://www.ebi.ac.uk/pdbsum/4z2f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z2f ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0M3KL31_9AGAM A0A0M3KL31_9AGAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Athelia rolfsii]] | | [[Category: Athelia rolfsii]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kantsadi, A L]] | + | [[Category: Kantsadi AL]] |
| - | [[Category: Leonidas, D D]] | + | [[Category: Leonidas DD]] |
| - | [[Category: Peppa, V I]] | + | [[Category: Peppa VI]] |
| - | [[Category: Carbohydrate-binding specificity]]
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| - | [[Category: Lectin]]
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| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
A0A0M3KL31_9AGAM
Publication Abstract from PubMed
SRL is a cell wall associated developmental-stage specific lectin secreted by Sclerotium rolfsii, a soil-born pathogenic fungus. SRL displays specificity for TF antigen (Galbeta1-->3GalNAc-alpha-Ser//Thr) expressed in all cancer types and has tumour suppressing effects in vivo. Considering the immense potential of SRL in cancer research, we have generated two variant gene constructs of SRL and expressed in E. coli to refine the sugar specificity and solubility by altering the surface charge. SSR1 and SSR2 are two different recombinant variants of SRL, both of which recognize TF antigen but only SSR1 binds to Tn antigen (GalNAcalpha-Ser/Thr). The glycan array analysis of the variants demonstrated that SSR1 recognizes TF antigen and their derivative with high affinity similar to SRL but showed highest affinity towards the sialylated Tn antigen, unlike SRL. The carbohydrate binding property of SSR2 remains unaltered compared to SRL. The crystal structures of the two variants were determined in free form and in complex with N-acetylglucosamine at 1.7 A and 1.6 A resolution, respectively. Structural analysis highlighted the structural basis of the fine carbohydrate specificity of the two SRL variants and results are in agreement with glycan array analysis.
Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants.,Peppa VI, Venkat H, Kantsadi AL, Inamdar SR, Bhat GG, Eligar S, Shivanand A, Chachadi VB, Satisha GJ, Swamy BM, Skamnaki VT, Zographos SE, Leonidas DD Molecules. 2015 Jun 12;20(6):10848-65. doi: 10.3390/molecules200610848. PMID:26076107[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Peppa VI, Venkat H, Kantsadi AL, Inamdar SR, Bhat GG, Eligar S, Shivanand A, Chachadi VB, Satisha GJ, Swamy BM, Skamnaki VT, Zographos SE, Leonidas DD. Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants. Molecules. 2015 Jun 12;20(6):10848-65. doi: 10.3390/molecules200610848. PMID:26076107 doi:http://dx.doi.org/10.3390/molecules200610848
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