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| | <StructureSection load='4z5v' size='340' side='right'caption='[[4z5v]], [[Resolution|resolution]] 3.05Å' scene=''> | | <StructureSection load='4z5v' size='340' side='right'caption='[[4z5v]], [[Resolution|resolution]] 3.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z5V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_A59 Murine hepatitis virus strain A59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z5V FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11142 CVMA5])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [https://pdbe.org/4z5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [https://www.ebi.ac.uk/pdbsum/4z5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z5v ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [http://pdbe.org/4z5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [http://www.ebi.ac.uk/pdbsum/4z5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z5v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5]] Not essential for virus replication in transformed murine cells. | + | [https://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5] Not essential for virus replication in transformed murine cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cvma5]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Huang, J H]] | + | [[Category: Murine hepatitis virus strain A59]] |
| - | [[Category: Peng, G Q]] | + | [[Category: Huang JH]] |
| - | [[Category: Sui, B K]] | + | [[Category: Peng GQ]] |
| - | [[Category: Zhao, L]] | + | [[Category: Sui BK]] |
| - | [[Category: Murine hepatitis virus]] | + | [[Category: Zhao L]] |
| - | [[Category: Ns2 protein]]
| + | |
| - | [[Category: Phosphodiesterase]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
NS2A_CVMA5 Not essential for virus replication in transformed murine cells.
Publication Abstract from PubMed
Prior studies have demonstrated that the mouse hepatitis virus A59 strain (MHV-A59) ns2 protein is a member of the 2H phosphoesterase family and exhibits 2',5'-phosphodiesterase (2',5'-PDE) activity. During the interferon antiviral response, ns2 cleaves 2',5'-oligoadenylate (2-5A), a key mediator of RNase L activation, thereby subverting the activation of RNase L and evading host innate immunity. However, the mechanism of 2-5A cleavage by ns2 remains unclear. Here, we present the crystal structure of the MHV ns2 PDE domain and demonstrate a PDE fold similar to that of the cellular protein, a kinase anchoring protein 7 central domain (AKAP7CD) and rotavirus VP3 carboxy-terminal domain (VP3-CTD). The structure displays a pair of strictly conserved HxS/Tx motifs and forms a deep positively charged catalytic groove with beta-sheets and an arginine containing loop (R-loop). These findings provide insight into the structural basis for 2-5A binding of MHV ns2.
Crystal structure of the mouse hepatitis virus ns2 phosphodiesterase domain that antagonizes RNase L activation.,Sui B, Huang J, Jha BK, Yin P, Zhou M, Fu ZF, Silverman RH, Weiss SR, Peng G, Zhao L J Gen Virol. 2016 Jan 12. doi: 10.1099/jgv.0.000395. PMID:26757803[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sui B, Huang J, Jha BK, Yin P, Zhou M, Fu ZF, Silverman RH, Weiss SR, Peng G, Zhao L. Crystal structure of the mouse hepatitis virus ns2 phosphodiesterase domain that antagonizes RNase L activation. J Gen Virol. 2016 Jan 12. doi: 10.1099/jgv.0.000395. PMID:26757803 doi:http://dx.doi.org/10.1099/jgv.0.000395
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