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4za6

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Revision as of 07:46, 10 May 2023

Structure of the R. erythropolis transcriptional repressor QsdR from TetR family

Structural highlights

4za6 is a 2 chain structure with sequence from Rhodococcus erythropolis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0C2W9F0_RHOER

Publication Abstract from PubMed

Quorum-quenching (QQ) are natural or engineered processes disrupting the quorum-sensing (QS) signalling which controls virulence and persistence (e.g. biofilm) in numerous bacteria. QQ involves different enzymes including lactonases, amidases, oxidases and reductases which degrade the QS molecules such as N-acylhomoserine lactones (NAHL). Rhodococcus erythropolis known to efficiently degrade NAHL is proposed as a biocontrol agent and a reservoir of QQ-enzymes for biotechnology. In R. erythropolis, regulation of QQ-enzymes remains unclear. In this work, we performed genome engineering on R. erythropolis, which is recalcitrant to reverse genetics, in order to investigate regulation of QQ-enzymes at a molecular and structural level with the aim to improve the QQ activity. Deep-sequencing of the R. erythropolis enhanced variants allowed identification of a punctual mutation in a key-transcriptional factor QsdR (Quorum sensing degradation Regulation) which regulates the sole QQ-lactonase QsdA identified so far. Using biophysical and structural studies on QsdR, we demonstrate that QQ activity can be improved by modifying the regulation of QQ-enzymes degrading QS signal. This modification requiring the change of only one amino-acid in a transcriptional factor leads to an enhanced R. erythropolis in which the QS-signal degradation pathway is strongly activated.

Natural Guided Genome Engineering Reveals Transcriptional Regulators Controlling Quorum-Sensing Signal Degradation.,El Sahili A, Kwasiborski A, Mothe N, Velours C, Legrand P, Morera S, Faure D PLoS One. 2015 Nov 10;10(11):e0141718. doi: 10.1371/journal.pone.0141718., eCollection 2015. PMID:26554837^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ El Sahili A, Kwasiborski A, Mothe N, Velours C, Legrand P, Morera S, Faure D. Natural Guided Genome Engineering Reveals Transcriptional Regulators Controlling Quorum-Sensing Signal Degradation. PLoS One. 2015 Nov 10;10(11):e0141718. doi: 10.1371/journal.pone.0141718., eCollection 2015. PMID:26554837 doi:http://dx.doi.org/10.1371/journal.pone.0141718

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4za6"

Categories: Large Structures | Rhodococcus erythropolis | El Sahili A | Morera S

@@ Line 3: / Line 3: @@
 <StructureSection load='4za6' size='340' side='right'caption='[[4za6]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4za6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZA6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4za6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZA6 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">QV65_28910 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4za6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4za6 OCA], [https://pdbe.org/4za6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4za6 RCSB], [https://www.ebi.ac.uk/pdbsum/4za6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4za6 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4za6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4za6 OCA], [http://pdbe.org/4za6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4za6 RCSB], [http://www.ebi.ac.uk/pdbsum/4za6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4za6 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0C2W9F0_RHOER A0A0C2W9F0_RHOER]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Mycobacterium erythropolis gray and thornton 1928]]
 [[Category: Large Structures]]
-[[Category: Morera, S]]
+[[Category: Rhodococcus erythropolis]]
-[[Category: Sahili, A El]]
+[[Category: El Sahili A]]
-[[Category: Nahl degradation]]
+[[Category: Morera S]]
-[[Category: Qsda transcriptional regulator]]
-[[Category: Quorum-sensing]]
-[[Category: Rhodococcus erythropolis r138]]
-[[Category: Signaling protein]]

4za6

From Proteopedia

Revision as of 07:46, 10 May 2023

Structure of the R. erythropolis transcriptional repressor QsdR from TetR family

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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