4zds

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(Difference between revisions)

Revision as of 07:50, 10 May 2023

Crystal Structure of core DNA binding domain of Arabidopsis Thaliana Transcription Factor Ethylene-Insensitive 3

Structural highlights

4zds is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EIN3_ARATH Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.^[1] ^[2]

Publication Abstract from PubMed

Gaseous hormone ethylene regulates numerous stress responses and developmental adaptations in plants by controlling gene expression via transcription factors ETHYLENE INSENSITIVE3 (EIN3) and EIN3-Like1 (EIL1). However, our knowledge regarding to the accurate definition of DNA-binding domains (DBDs) within EIN3 and also the mechanism of specific DNA recognition by EIN3 is limited. Here, we identify EIN3 82-352 and 174-306 as the optimal and core DBDs, respectively. Results from systematic biochemical analyses reveal that both the number of EIN3-binding sites (EBSs) and the spacing length between two EBSs affect the binding affinity of EIN3; accordingly, a new DNA probe which has higher affinity with EIN3 than ERF1 is also designed. Furthermore, we show that palindromic repeat sequences in ERF1 promoter are not necessary for EIN3 binding. Finally, we provide, to our knowledge, the first crystal structure of EIN3 core DBD, which contains amino acid residues essential for DNA binding and signaling. Collectively, these data suggest the detailed mechanism of DNA recognition by EIN3 and provide an in-depth view at molecular level for the transcriptional regulation mediated by EIN3.

Biochemical and Structural Insights into the Mechanism of DNA Recognition by Arabidopsis ETHYLENE INSENSITIVE3.,Song J, Zhu C, Zhang X, Wen X, Liu L, Peng J, Guo H, Yi C PLoS One. 2015 Sep 9;10(9):e0137439. doi: 10.1371/journal.pone.0137439., eCollection 2015. PMID:26352699^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker JR. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell. 1997 Jun 27;89(7):1133-44. PMID:9215635
↑ Solano R, Stepanova A, Chao Q, Ecker JR. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev. 1998 Dec 1;12(23):3703-14. PMID:9851977
↑ Song J, Zhu C, Zhang X, Wen X, Liu L, Peng J, Guo H, Yi C. Biochemical and Structural Insights into the Mechanism of DNA Recognition by Arabidopsis ETHYLENE INSENSITIVE3. PLoS One. 2015 Sep 9;10(9):e0137439. doi: 10.1371/journal.pone.0137439., eCollection 2015. PMID:26352699 doi:http://dx.doi.org/10.1371/journal.pone.0137439

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4zds"

@@ Line 3: / Line 3: @@
 <StructureSection load='4zds' size='340' side='right'caption='[[4zds]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zds]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZDS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zds]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZDS FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zds OCA], [http://pdbe.org/4zds PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zds RCSB], [http://www.ebi.ac.uk/pdbsum/4zds PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zds ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zds OCA], [https://pdbe.org/4zds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zds RCSB], [https://www.ebi.ac.uk/pdbsum/4zds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zds ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/EIN3_ARATH EIN3_ARATH]] Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.<ref>PMID:9215635</ref> <ref>PMID:9851977</ref>
+[https://www.uniprot.org/uniprot/EIN3_ARATH EIN3_ARATH] Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.<ref>PMID:9215635</ref> <ref>PMID:9851977</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 [[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Guo, H]]
+[[Category: Guo H]]
-[[Category: Liu, L]]
+[[Category: Liu L]]
-[[Category: Peng, J]]
+[[Category: Peng J]]
-[[Category: Song, J]]
+[[Category: Song J]]
-[[Category: Wen, X]]
+[[Category: Wen X]]
-[[Category: Yi, C]]
+[[Category: Yi C]]
-[[Category: Zhang, X]]
+[[Category: Zhang X]]
-[[Category: Zhu, C]]
+[[Category: Zhu C]]
-[[Category: Dna binding domain transcription factor]]
-[[Category: Transcription activator]]

4zds

From Proteopedia

Revision as of 07:50, 10 May 2023

Crystal Structure of core DNA binding domain of Arabidopsis Thaliana Transcription Factor Ethylene-Insensitive 3

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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