1ksq
From Proteopedia
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'''NMR Study of the Third TB Domain from Latent Transforming Growth Factor-beta Binding Protein-1''' | '''NMR Study of the Third TB Domain from Latent Transforming Growth Factor-beta Binding Protein-1''' | ||
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[[Category: Rifkin, D B.]] | [[Category: Rifkin, D B.]] | ||
[[Category: Yuan, X.]] | [[Category: Yuan, X.]] | ||
| - | [[Category: | + | [[Category: Leary, J M.O.]] |
| - | [[Category: | + | [[Category: Lap]] |
| - | [[Category: | + | [[Category: Latency associated propeptide]] |
| - | [[Category: | + | [[Category: Latent transforming growth factor-beta binding protein-1]] |
| - | [[Category: | + | [[Category: Ltbp-1]] |
| - | [[Category: | + | [[Category: Nmr structure]] |
| - | [[Category: | + | [[Category: Tb domain]] |
| - | [[Category: | + | [[Category: Tgf-beta]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:07:35 2008'' | |
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Revision as of 20:07, 2 May 2008
NMR Study of the Third TB Domain from Latent Transforming Growth Factor-beta Binding Protein-1
Overview
Almost all TGF-beta is secreted as part of a large latent complex. This complex is formed from three molecules, a latent transforming growth factor-beta binding protein (LTBP), which plays roles in targeting and activation, a latency associated peptide (LAP), which regulates latency, and the TGF-beta cytokine. LAP is the TGF-beta pro-peptide that is cleaved intracellularly prior to secretion, and TGF-beta binds non-covalently to LAP. Formation of the large latent complex is important for the efficient secretion of TGF-beta. Previous studies have revealed that the LTBP-LAP interaction is mediated by intracellular exchange of a single disulphide bond within the third, and only the third, TB domain (TB3) with LAP. We have previously reported the structure of a homologous TB domain from fibrillin-1. However, TB3 contains a two amino acid insertion, not found in fibrillin-1 TB domains, which is not amenable to molecular modelling. In order to clarify the basis of TB domain function, we have determined the solution NMR structure of TB3(LTBP1). Comparison with the fibrillin-1 TB domain reveals that the two-residue insertion is associated with a significant increase in solvent accessibility of one of the disulphide bonds (linking the second and sixth cysteine residues). Site-directed mutagenesis and NMR studies indicate that this is the only disulphide bond that can be removed without perturbing the TB domain fold. Furthermore, a ring of negatively charged residues has been identified that surrounds this disulphide bond. Homology modelling suggests that the surface properties of TB3 domains from different LTBP isoforms correlate with binding activities. This research provides testable hypotheses regarding the molecular basis of complex formation between LTBPs and LAPs.
About this Structure
1KSQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the third TB domain from LTBP1 provides insight into assembly of the large latent complex that sequesters latent TGF-beta., Lack J, O'Leary JM, Knott V, Yuan X, Rifkin DB, Handford PA, Downing AK, J Mol Biol. 2003 Nov 21;334(2):281-91. PMID:14607119 Page seeded by OCA on Fri May 2 23:07:35 2008
