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| | <StructureSection load='4zlf' size='340' side='right'caption='[[4zlf]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='4zlf' size='340' side='right'caption='[[4zlf]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbulbifer_degradans_2-40 Microbulbifer degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zle|4zle]], [[4zlg|4zlg]], [[4zli|4zli]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [https://pdbe.org/4zlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [https://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cep94B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203122 Microbulbifer degradans 2-40])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [http://pdbe.org/4zlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [http://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q21MB1_SACD2 Q21MB1_SACD2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cellobionic acid phosphorylase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Microbulbifer degradans 2-40]] | + | [[Category: Saccharophagus degradans 2-40]] |
| - | [[Category: Arakawa, T]] | + | [[Category: Arakawa T]] |
| - | [[Category: Fushinobu, S]] | + | [[Category: Fushinobu S]] |
| - | [[Category: Nam, Y W]] | + | [[Category: Nam YW]] |
| - | [[Category: Glycoside hydrolase family 94]]
| + | |
| - | [[Category: Intracellular enzyme]]
| + | |
| - | [[Category: Oxidative cellulose degradation system]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q21MB1_SACD2
Publication Abstract from PubMed
The microbial oxidative cellulose degradation system is attracting significant research attention after the recent discovery of lytic polysaccharide mono-oxygenases. A primary product of the oxidative and hydrolytic cellulose degradation system is cellobionic acid (CbA), the aldonic acid form of cellobiose. We previously demonstrated that the intracellular enzyme belonging to glycoside hydrolase (GH) family 94 from cellulolytic fungus and bacterium is cellobionic acid phosphorylase (CBAP), which catalyzes reversible phosphorolysis of CbA into glucose 1-phosphate and gluconic acid (GlcA). In this report we describe the biochemical characterization and the three-dimensional structure of CBAP from the marine cellulolytic bacterium Saccharophagus degradans. Structures of ligand-free and complex forms with CbA, GlcA, and a synthetic disaccharide product from glucuronic acid (GlcUA) were determined at resolutions of up to 1.6 A. The active site is located near the dimer interface. At subsite +1, the carboxylate group of GlcA and CbA is recognized by Arg-609 and Lys-613. Additionally, one residue from the neighboring protomer (Gln-190) is involved in the carboxylate recognition of GlcA. A mutational analysis indicated that these residues are critical for the binding and catalysis of the aldonic and uronic acid acceptors GlcA and GlcUA. Structural and sequence comparisons with other GH94 phosphorylases revealed that CBAPs have a unique subsite +1 with a distinct amino acid residue conservation pattern at this site. This study provides molecular insight into the energetically efficient metabolic pathway of oxidized sugars that links the oxidative cellulolytic pathway to the glycolytic and pentose phosphate pathways in cellulolytic microbes.
Crystal structure and substrate recognition of cellobionic acid phosphorylase playing a key role in oxidative cellulose degradation by microbes.,Nam YW, Nihira T, Arakawa T, Saito Y, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2015 Jun 3. pii: jbc.M115.664664. PMID:26041776[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nam YW, Nihira T, Arakawa T, Saito Y, Kitaoka M, Nakai H, Fushinobu S. Crystal structure and substrate recognition of cellobionic acid phosphorylase playing a key role in oxidative cellulose degradation by microbes. J Biol Chem. 2015 Jun 3. pii: jbc.M115.664664. PMID:26041776 doi:http://dx.doi.org/10.1074/jbc.M115.664664
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