1ktx
From Proteopedia
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'''KALIOTOXIN (1-37) SHOWS STRUCTURAL DIFFERENCES WITH RELATED POTASSIUM CHANNEL BLOCKERS''' | '''KALIOTOXIN (1-37) SHOWS STRUCTURAL DIFFERENCES WITH RELATED POTASSIUM CHANNEL BLOCKERS''' | ||
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[[Category: Romi, R.]] | [[Category: Romi, R.]] | ||
[[Category: Szendefi, S.]] | [[Category: Szendefi, S.]] | ||
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Revision as of 20:10, 2 May 2008
KALIOTOXIN (1-37) SHOWS STRUCTURAL DIFFERENCES WITH RELATED POTASSIUM CHANNEL BLOCKERS
Overview
The three-dimensional structure of kaliotoxin (1-37), KTX(1-37), a toxin from the scorpion Androctonus mauretanicus mauretanicus that blocks calcium-dependent potassium channels, has been determined by NMR. This toxin is homologous with other scorpion toxins such as charybdotoxin (ChTX) or iberiotoxin (IbTX) for which the structures are already known, but the presence of prolines in the expected alpha-helical region suggested that there may be some major difference in the structure of KTX that could be related to its different selectivity. Proline residues are also found in the homologous region of other scorpion toxins such as noxiustoxin or margatoxin. Our results indicate that KTX(1-37) contains the same sequence of secondary structure elements as ChTX but that the helical region is shorter and distorted due to the presence of two prolines. The distortion consists of a bending in the alpha-helix and in the presence of a 3(10) helix turn in the last three residues. Furthermore, the increased length of the extended structure preceding the helix favors a different packing of this part of the molecule with respect to the secondary structure elements. This change in folding modifies the accessibility of the conserved 27Lys which is known, from mutation studies, to be involved in channel blocking by ChTX.
About this Structure
1KTX is a Single protein structure of sequence from Androctonus mauretanicus mauretanicus. Full crystallographic information is available from OCA.
Reference
Kaliotoxin (1-37) shows structural differences with related potassium channel blockers., Fernandez I, Romi R, Szendeffy S, Martin-Eauclaire MF, Rochat H, Van Rietschoten J, Pons M, Giralt E, Biochemistry. 1994 Nov 29;33(47):14256-63. PMID:7524673 Page seeded by OCA on Fri May 2 23:10:04 2008
