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| | ==PlyCB mutant R66E== | | ==PlyCB mutant R66E== |
| - | <StructureSection load='4zrz' size='340' side='right' caption='[[4zrz]], [[Resolution|resolution]] 1.72Å' scene=''> | + | <StructureSection load='4zrz' size='340' side='right'caption='[[4zrz]], [[Resolution|resolution]] 1.72Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zrz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_phage_c1 Streptococcus phage c1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zrz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_virus_C1 Streptococcus virus C1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZRZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f87|4f87]], [[4f88|4f88]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zrz OCA], [https://pdbe.org/4zrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zrz RCSB], [https://www.ebi.ac.uk/pdbsum/4zrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zrz ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=230871 Streptococcus phage C1])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zrz OCA], [http://pdbe.org/4zrz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zrz RCSB], [http://www.ebi.ac.uk/pdbsum/4zrz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zrz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7Y3F3_9CAUD Q7Y3F3_9CAUD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Streptococcus phage c1]] | + | [[Category: Large Structures]] |
| - | [[Category: Gallagher, D T]] | + | [[Category: Streptococcus virus C1]] |
| - | [[Category: Nelson, D C]] | + | [[Category: Gallagher DT]] |
| - | [[Category: Shen, Y]] | + | [[Category: Nelson DC]] |
| - | [[Category: Antimicrobial protein]] | + | [[Category: Shen Y]] |
| - | [[Category: Bacteriocidal]]
| + | |
| - | [[Category: Bacteriophage lysin]]
| + | |
| - | [[Category: Cell-binding subunit]]
| + | |
| - | [[Category: Cell-wall-cutting]]
| + | |
| - | [[Category: Octameric]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Function
Q7Y3F3_9CAUD
Publication Abstract from PubMed
PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PlyC holoenzyme, mediated by its PlyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using model membranes establish that PlyCB interacts strongly with phosphatidylserine (PS), whereas its interaction with other lipids is weak, suggesting specificity for PS as its cellular receptor. Neutron reflection further substantiates that PlyC penetrates bilayers above a PS threshold concentration. Crystallography and docking studies identify key residues that mediate PlyCB-PS interactions, which are validated by site-directed mutagenesis. This is the first report that a native endolysin can traverse epithelial membranes, thus substantiating the potential of PlyC as an antimicrobial for Spy in the extracellular and intracellular milieu and as a scaffold for engineering other functionalities.
A bacteriophage endolysin that eliminates intracellular streptococci.,Shen Y, Barros M, Vennemann T, Gallagher DT, Yin Y, Linden SB, Heselpoth RD, Spencer DJ, Donovan DM, Moult J, Fischetti VA, Heinrich F, Losche M, Nelson DC Elife. 2016 Mar 15;5. doi: 10.7554/eLife.13152. PMID:26978792[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shen Y, Barros M, Vennemann T, Gallagher DT, Yin Y, Linden SB, Heselpoth RD, Spencer DJ, Donovan DM, Moult J, Fischetti VA, Heinrich F, Losche M, Nelson DC. A bacteriophage endolysin that eliminates intracellular streptococci. Elife. 2016 Mar 15;5. doi: 10.7554/eLife.13152. PMID:26978792 doi:http://dx.doi.org/10.7554/eLife.13152
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