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| | <StructureSection load='4zyj' size='340' side='right'caption='[[4zyj]], [[Resolution|resolution]] 2.74Å' scene=''> | | <StructureSection load='4zyj' size='340' side='right'caption='[[4zyj]], [[Resolution|resolution]] 2.74Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zyj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1799 As 4.1799]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZYJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zyj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZYJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zxv|4zxv]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyj OCA], [https://pdbe.org/4zyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zyj RCSB], [https://www.ebi.ac.uk/pdbsum/4zyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyj ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnmZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1950 AS 4.1799])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-evernosamine_nitrososynthase L-evernosamine nitrososynthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.187 1.14.13.187] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyj OCA], [http://pdbe.org/4zyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zyj RCSB], [http://www.ebi.ac.uk/pdbsum/4zyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyj ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0R4I990_STRPE A0A0R4I990_STRPE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: As 4 1799]] | |
| - | [[Category: L-evernosamine nitrososynthase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sartor, L M]] | + | [[Category: Streptomyces peucetius]] |
| - | [[Category: Vey, J L]] | + | [[Category: Sartor LM]] |
| - | [[Category: Acyl-coa dehydrogenase]] | + | [[Category: Vey JL]] |
| - | [[Category: Aminosugar]]
| + | |
| - | [[Category: Cis-peptide]]
| + | |
| - | [[Category: Flavin]]
| + | |
| - | [[Category: Flavin monooxygenase]]
| + | |
| - | [[Category: Nitrososynthase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
A0A0R4I990_STRPE
Publication Abstract from PubMed
The anthracyclines are a class of highly effective natural product chemotherapeutics and are used to treat a range of cancers, including leukemia. The toxicity of the anthracyclines has stimulated efforts to further diversify the scaffold of the natural product, which has led to renewed interest in the biosynthetic pathway responsible for the formation and modification of this family of molecules. DnmZ is an N-hydroxylating flavin monooxygenase (a nitrososynthase) that catalyzes the oxidation of the exocyclic amine of the sugar nucleotide dTDP-L-epi-vancosamine to its nitroso form. Its specific role in the anthracycline biosynthetic pathway involves the synthesis of the seven-carbon acetal moiety attached to C4 of L-daunosamine observed in the anthracycline baumycin. Here, X-ray crystallography was used to elucidate the three-dimensional structure of DnmZ. Two crystal structures of DnmZ were yielded: that of the enzyme alone, solved to 3.00 A resolution, and that of the enzyme in complex with thymidine diphosphate, the nucleotide carrier portion of the substrate, solved to 2.74 A resolution. These models add insights into the structural features involved in substrate specificity and conformational changes involved in thymidine diphosphate binding by the nitrososynthases.
Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline biosynthetic pathway.,Sartor L, Ibarra C, Al-Mestarihi A, Bachmann BO, Vey JL Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1205-14. doi:, 10.1107/S2053230X15014272. Epub 2015 Sep 23. PMID:26457508[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sartor L, Ibarra C, Al-Mestarihi A, Bachmann BO, Vey JL. Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline biosynthetic pathway. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1205-14. doi:, 10.1107/S2053230X15014272. Epub 2015 Sep 23. PMID:26457508 doi:http://dx.doi.org/10.1107/S2053230X15014272
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