1kvm
From Proteopedia
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[[Image:1kvm.gif|left|200px]] | [[Image:1kvm.gif|left|200px]] | ||
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'''X-ray Crystal Structure of AmpC WT beta-Lactamase in Complex with Covalently Bound Cephalothin''' | '''X-ray Crystal Structure of AmpC WT beta-Lactamase in Complex with Covalently Bound Cephalothin''' | ||
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[[Category: Shoichet, B K.]] | [[Category: Shoichet, B K.]] | ||
[[Category: Trehan, I.]] | [[Category: Trehan, I.]] | ||
| - | [[Category: | + | [[Category: Acyl-enzyme complex]] |
| - | [[Category: | + | [[Category: Amide hydrolase]] |
| - | [[Category: | + | [[Category: Beta-lactamase]] |
| - | [[Category: | + | [[Category: Cephalothin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:13:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:13, 2 May 2008
X-ray Crystal Structure of AmpC WT beta-Lactamase in Complex with Covalently Bound Cephalothin
Overview
Beta-lactamases hydrolyze beta-lactam antibiotics and are the leading cause of bacterial resistance to these drugs. Although beta-lactamases have been extensively studied, structures of the substrate-enzyme and product-enzyme complexes have proven elusive. Here, the structure of a mutant AmpC in complex with the beta-lactam cephalothin in its substrate and product forms was determined by X-ray crystallography to 1.53 A resolution. The acyl-enzyme intermediate between AmpC and cephalothin was determined to 2.06 A resolution. The ligand undergoes a dramatic conformational change as the reaction progresses, with the characteristic six-membered dihydrothiazine ring of cephalothin rotating by 109 degrees. These structures correspond to all three intermediates along the reaction path and provide insight into substrate recognition, catalysis, and product expulsion.
About this Structure
1KVM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural milestones in the reaction pathway of an amide hydrolase: substrate, acyl, and product complexes of cephalothin with AmpC beta-lactamase., Beadle BM, Trehan I, Focia PJ, Shoichet BK, Structure. 2002 Mar;10(3):413-24. PMID:12005439 Page seeded by OCA on Fri May 2 23:13:22 2008
