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5a30

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Revision as of 04:36, 25 May 2023

Crystal structure of mtPAP N472D mutant in complex with ATPgammaS

Structural highlights

5a30 is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F1NBW0_CHICK

Publication Abstract from PubMed

Polyadenylation, performed by poly(A) polymerases (PAPs), is a ubiquitous post-transcriptional modification that plays key roles in multiple aspects of RNA metabolism. Although cytoplasmic and nuclear PAPs have been studied extensively, the mechanism by which mitochondrial PAP (mtPAP) selects adenosine triphosphate over other nucleotides is unknown. Furthermore, mtPAP is unique because it acts as a dimer. However, mtPAP's dimerization requirement remains enigmatic. Here, we show the structural basis for mtPAP's nucleotide selectivity, dimerization and catalysis. Our structures reveal an intricate dimerization interface that features an RNA-recognition module formed through strand complementation. Further, we propose the structural basis for the N478D mutation that drastically reduces the length of poly(A) tails on mitochondrial mRNAs in patients with spastic ataxia 4 (SPAX4), a severe and progressive neurodegenerative disease.

Structure of mitochondrial poly(A) RNA polymerase reveals the structural basis for dimerization, ATP selectivity and the SPAX4 disease phenotype.,Lapkouski M, Hallberg BM Nucleic Acids Res. 2015 Aug 28. pii: gkv861. PMID:26319014^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lapkouski M, Hallberg BM. Structure of mitochondrial poly(A) RNA polymerase reveals the structural basis for dimerization, ATP selectivity and the SPAX4 disease phenotype. Nucleic Acids Res. 2015 Aug 28. pii: gkv861. PMID:26319014 doi:http://dx.doi.org/10.1093/nar/gkv861

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a30"

Categories: Gallus gallus | Large Structures | Hallberg BM | Lapkouski M

@@ Line 3: / Line 3: @@
 <StructureSection load='5a30' size='340' side='right'caption='[[5a30]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a30]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A30 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a30]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A30 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a2v|5a2v]], [[5a2w|5a2w]], [[5a2x|5a2x]], [[5a2y|5a2y]], [[5a2z|5a2z]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a30 OCA], [https://pdbe.org/5a30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a30 RCSB], [https://www.ebi.ac.uk/pdbsum/5a30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a30 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a30 OCA], [http://pdbe.org/5a30 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a30 RCSB], [http://www.ebi.ac.uk/pdbsum/5a30 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a30 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/F1NBW0_CHICK F1NBW0_CHICK]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Chick]]
+[[Category: Gallus gallus]]
 [[Category: Large Structures]]
-[[Category: Hallberg, B M]]
+[[Category: Hallberg BM]]
-[[Category: Lapkouski, M]]
+[[Category: Lapkouski M]]
-[[Category: Unknown function]]

5a30

From Proteopedia

Revision as of 04:36, 25 May 2023

Crystal structure of mtPAP N472D mutant in complex with ATPgammaS

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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