5a79

From Proteopedia

(Difference between revisions)

Revision as of 04:42, 25 May 2023

Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM

5a79, resolution 4.10Å

Structural highlights

5a79 is a 2 chain structure with sequence from Barley stripe mosaic virus and Tobacco mosaic virus strain OM. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_BSMV Capsid protein self-assembles to form rod-shaped virions about 25 nm in diameter with a central canal enclosing the viral genomic RNA.

Publication Abstract from PubMed

Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report the first high-resolution structure of BSMV at 4.1 A obtained by cryo-electron microscopy. We discovered that BSMV forms two types of virion that differ in the number of coat protein (CP) subunits per turn and interactions between the CP subunits. While BSMV and tobacco mosaic virus CP subunits have a similar fold and interact with RNA using conserved residues, the axial contacts between the CP of these two viral groups are considerably different. BSMV CP subunits lack substantial axial contacts and are held together by a previously unobserved lateral contact formed at the virion surface via an interacting loop, which protrudes from the CP hydrophobic core to the adjacent CP subunit. These data provide an insight into diversity in structural organization of helical viruses.

Novel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy.,Clare DK, Pechnikova EV, Skurat EV, Makarov VV, Sokolova OS, Solovyev AG, Orlova EV Structure. 2015 Aug 13. pii: S0969-2126(15)00287-7. doi:, 10.1016/j.str.2015.06.028. PMID:26278173^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Clare DK, Pechnikova EV, Skurat EV, Makarov VV, Sokolova OS, Solovyev AG, Orlova EV. Novel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy. Structure. 2015 Aug 13. pii: S0969-2126(15)00287-7. doi:, 10.1016/j.str.2015.06.028. PMID:26278173 doi:http://dx.doi.org/10.1016/j.str.2015.06.028

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a79"

@@ Line 3: / Line 3: @@
 <SX load='5a79' size='340' side='right' viewer='molstar' caption='[[5a79]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a79]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bsmv Bsmv] and [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus_(strain_om) Tobacco mosaic virus (strain om)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A79 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5A79 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a79]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Barley_stripe_mosaic_virus Barley stripe mosaic virus] and [https://en.wikipedia.org/wiki/Tobacco_mosaic_virus_strain_OM Tobacco mosaic virus strain OM]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A79 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a7a|5a7a]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a79 OCA], [https://pdbe.org/5a79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a79 RCSB], [https://www.ebi.ac.uk/pdbsum/5a79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a79 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5a79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a79 OCA], [http://pdbe.org/5a79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a79 RCSB], [http://www.ebi.ac.uk/pdbsum/5a79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a79 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CAPSD_BSMV CAPSD_BSMV]] Capsid protein self-assembles to form rod-shaped virions about 25 nm in diameter with a central canal enclosing the viral genomic RNA.
+[https://www.uniprot.org/uniprot/CAPSD_BSMV CAPSD_BSMV] Capsid protein self-assembles to form rod-shaped virions about 25 nm in diameter with a central canal enclosing the viral genomic RNA.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 19: @@
 ==See Also==
+*[[Tobacco Mosaic Virus|Tobacco Mosaic Virus]]
 *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 == References ==
@@ Line 25: / Line 25: @@
 __TOC__
 </SX>
-[[Category: Bsmv]]
+[[Category: Barley stripe mosaic virus]]
 [[Category: Large Structures]]
-[[Category: Clare, D K]]
+[[Category: Tobacco mosaic virus strain OM]]
-[[Category: Makarov, V]]
+[[Category: Clare DK]]
-[[Category: Orlova, E V]]
+[[Category: Makarov V]]
-[[Category: Pechnikova, E]]
+[[Category: Pechnikova E]]
-[[Category: Skurat, E]]
+[[Category: Skurat E]]
-[[Category: Sokolova, O S]]
+[[Category: Sokolova OS]]
-[[Category: Solovyev, A G]]
+[[Category: Solovyev AG]]
-[[Category: Cryo-em]]
+[[Category: V Orlova E]]
-[[Category: Helical]]
-[[Category: Image processing]]
-[[Category: Msa]]
-[[Category: Virus]]

5a79

From Proteopedia

Revision as of 04:42, 25 May 2023

Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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