1kwf
From Proteopedia
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'''Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate''' | '''Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate''' | ||
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[[Category: Lascombe, M B.]] | [[Category: Lascombe, M B.]] | ||
[[Category: Souchon, H.]] | [[Category: Souchon, H.]] | ||
| - | [[Category: | + | [[Category: Atomic resolution]] |
| - | [[Category: | + | [[Category: Cellulase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Inverting glycosidase]] |
| - | [[Category: | + | [[Category: Protein-carbohydrate interaction]] |
| - | [[Category: | + | [[Category: Reaction mechanism]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:15:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:15, 2 May 2008
Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate
Overview
The crystal structure of Clostridium thermocellum endoglucanase CelA in complex with cellopentaose has been determined at 0.94 A resolution. The oligosaccharide occupies six D-glucosyl-binding subsites, three on either side of the scissile glycosidic linkage. The substrate and product of the reaction occupy different positions at the reducing end of the cleft, where an extended array of hydrogen-bonding interactions with water molecules fosters the departure of the leaving group. Severe torsional strain upon the bound substrate forces a distorted boat(2,5) B conformation for the glucosyl residue bound at subsite -1, which facilitates the formation of an oxocarbenium ion intermediate and might favor the breakage of the sugar ring concomitant with catalysis.
About this Structure
1KWF is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.
Reference
Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate., Guerin DM, Lascombe MB, Costabel M, Souchon H, Lamzin V, Beguin P, Alzari PM, J Mol Biol. 2002 Mar 8;316(5):1061-9. PMID:11884144 Page seeded by OCA on Fri May 2 23:15:06 2008
