5afe

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<StructureSection load='5afe' size='340' side='right'caption='[[5afe]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='5afe' size='340' side='right'caption='[[5afe]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5afe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AFE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AFE FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5afe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_cellulosolvens Eubacterium cellulosolvens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AFE FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5afe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5afe OCA], [https://pdbe.org/5afe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5afe RCSB], [https://www.ebi.ac.uk/pdbsum/5afe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5afe ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5afe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5afe OCA], [http://pdbe.org/5afe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5afe RCSB], [http://www.ebi.ac.uk/pdbsum/5afe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5afe ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q3LHN3_EUBCE Q3LHN3_EUBCE]
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The rumen anaerobic cellulolytic bacterium Eubacterium cellulosolvens produces a large range of cellulases and hemicellulases responsible for the efficient hydrolysis of plant cell wall polysaccharides. One of these enzymes, endoglucanase Cel5A, comprises a tandemly repeated carbohydrate-binding module (CBM65) fused to a glycoside hydrolase family 5 (Cel5A) catalytic domain, joined by flexible linker sequences. The second carbohydrate-binding module located at the C-terminus side of the endoglucanase (CBM65B) has been co-crystallized with either cellohexaose or xyloglucan heptasaccharide. The crystals belong to the hexagonal space group P6(5) and tetragonal space group P4(3)2(1)2, containing a single molecule in the asymmetric unit. The structures of CBM65B have been solved by molecular replacement.
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Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.,Venditto I, Basle A, Luis AS, Temple MJ, Ferreira LM, Fontes CM, Gilbert HJ, Najmudin S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):191-4. doi:, 10.1107/S1744309113001620. Epub 2013 Jan 31. PMID:23385766<ref>PMID:23385766</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5afe" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cellulase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Fontes, C M.G A]]
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[[Category: Fontes CMGA]]
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[[Category: Gilbert, H J]]
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[[Category: Gilbert HJ]]
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[[Category: Najmudin, S]]
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[[Category: Najmudin S]]
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[[Category: Venditto, I]]
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[[Category: Venditto I]]
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[[Category: Carbohydrate binding module]]
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[[Category: Cbm65b]]
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[[Category: Endoglucanase cel5a]]
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[[Category: Eubacterium cellulosolven]]
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[[Category: Hydrolase]]
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[[Category: Plant cell wall degradation]]
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[[Category: Xylogucan heptasaccharide]]
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Revision as of 04:55, 25 May 2023

Medium Resolution structure of the C-terminal family 65 Carbohydrate Binding Module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens with a bound xyloglucan heptasaccharide (XXXG)

PDB ID 5afe

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