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5ahn

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Revision as of 05:00, 25 May 2023

IMP-bound form of the D199N mutant of IMPDH from Pseudomonas aeruginosa

Structural highlights

5ahn is a 1 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HXM5_PSEAE Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity).[HAMAP-Rule:MF_01964]

Publication Abstract from PubMed

Inosine-5'-monophosphate dehydrogenases (IMPDHs), which are the rate-limiting enzymes in guanosine-nucleotide biosynthesis, are important therapeutic targets. Despite in-depth functional and structural characterizations of various IMPDHs, the role of the Bateman domain containing two CBS motifs remains controversial. Their involvement in the allosteric regulation of Pseudomonas aeruginosa IMPDH by Mg-ATP has recently been reported. To better understand the function of IMPDH and the importance of the CBS motifs, the structure of a variant devoid of these modules (DeltaCBS) was solved at high resolution in the apo form and in complex with IMP. In addition, a single amino-acid substitution variant, D199N, was also structurally characterized: the mutation corresponds to the autosomal dominant mutant D226N of human IMPDH1, which is responsible for the onset of the retinopathy adRP10. These new structures shed light onto the possible mechanism of regulation of the IMPDH enzymatic activity. In particular, three conserved loops seem to be key players in this regulation as they connect the tetramer-tetramer interface with the active site and show significant modification upon substrate binding.

Crystallographic studies of two variants of Pseudomonas aeruginosa IMPDH with impaired allosteric regulation.,Labesse G, Alexandre T, Gelin M, Haouz A, Munier-Lehmann H Acta Crystallogr D Biol Crystallogr. 2015 Sep 1;71(Pt 9):1890-9. doi:, 10.1107/S1399004715013115. Epub 2015 Aug 25. PMID:26327379^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Labesse G, Alexandre T, Gelin M, Haouz A, Munier-Lehmann H. Crystallographic studies of two variants of Pseudomonas aeruginosa IMPDH with impaired allosteric regulation. Acta Crystallogr D Biol Crystallogr. 2015 Sep 1;71(Pt 9):1890-9. doi:, 10.1107/S1399004715013115. Epub 2015 Aug 25. PMID:26327379 doi:http://dx.doi.org/10.1107/S1399004715013115

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ahn"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ahn' size='340' side='right'caption='[[5ahn]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ahn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AHN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ahn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AHN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ahl|5ahl]], [[5ahm|5ahm]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ahn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ahn OCA], [https://pdbe.org/5ahn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ahn RCSB], [https://www.ebi.ac.uk/pdbsum/5ahn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ahn ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/IMP_dehydrogenase IMP dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.205 1.1.1.205] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ahn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ahn OCA], [http://pdbe.org/5ahn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ahn RCSB], [http://www.ebi.ac.uk/pdbsum/5ahn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ahn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Q9HXM5_PSEAE Q9HXM5_PSEAE]] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity).[HAMAP-Rule:MF_01964]
+[https://www.uniprot.org/uniprot/Q9HXM5_PSEAE Q9HXM5_PSEAE] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity).[HAMAP-Rule:MF_01964]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: IMP dehydrogenase]]
 [[Category: Large Structures]]
-[[Category: Pseae]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Alexandre, T]]
+[[Category: Alexandre T]]
-[[Category: Gelin, M]]
+[[Category: Gelin M]]
-[[Category: Haouz, A]]
+[[Category: Haouz A]]
-[[Category: Labesse, G]]
+[[Category: Labesse G]]
-[[Category: Munier-Lehmann, H]]
+[[Category: Munier-Lehmann H]]
-[[Category: Allosteric regulation nucleotide metabolism]]
-[[Category: Cbs module]]
-[[Category: Oxidoreductase]]
-[[Category: Point mutant]]

5ahn

From Proteopedia

Revision as of 05:00, 25 May 2023

IMP-bound form of the D199N mutant of IMPDH from Pseudomonas aeruginosa

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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