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| | ==N-TYPE CALCIUM CHANNEL BLOCKER, OMEGA-CONOTOXIN MVIIA NMR, 15 STRUCTURES== | | ==N-TYPE CALCIUM CHANNEL BLOCKER, OMEGA-CONOTOXIN MVIIA NMR, 15 STRUCTURES== |
| - | <StructureSection load='1mvj' size='340' side='right'caption='[[1mvj]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='1mvj' size='340' side='right'caption='[[1mvj]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1mvj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Const Const]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1mvj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_striatus Conus striatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVJ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvj OCA], [https://pdbe.org/1mvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvj RCSB], [https://www.ebi.ac.uk/pdbsum/1mvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvj ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvj OCA], [https://pdbe.org/1mvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvj RCSB], [https://www.ebi.ac.uk/pdbsum/1mvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/CO16B_CONST CO16B_CONST]] Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This toxin blocks N-, P- and Q-type calcium channels.
| + | [https://www.uniprot.org/uniprot/O16B_CONST O16B_CONST] Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This toxin blocks N-, P- and Q-type calcium channels.<ref>PMID:1390774</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Const]] | + | [[Category: Conus striatus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alewood, P F]] | + | [[Category: Alewood PF]] |
| - | [[Category: Craik, D J]] | + | [[Category: Craik DJ]] |
| - | [[Category: Lewis, R J]] | + | [[Category: Lewis RJ]] |
| - | [[Category: Nielsen, K J]] | + | [[Category: Nielsen KJ]] |
| - | [[Category: Thomas, L]] | + | [[Category: Thomas L]] |
| - | [[Category: Conus striatus peptide specific to p/q-type voltage sensitive calcium channel]]
| + | |
| - | [[Category: Neurotoxin]]
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| Structural highlights
Function
O16B_CONST Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This toxin blocks N-, P- and Q-type calcium channels.[1]
Publication Abstract from PubMed
The omega-conotoxins are a set of structurally related peptides that have a wide range of specificities for different subtypes of the voltage-sensitive calcium channel (VSCC). To understand their VSCC subtype differentiation we studied the structure of two naturally occurring omega-conotoxins, MVIIA (specific to N-type) and SVIB (specific to P/Q-type) and a synthetic hybrid, SNX-202, which has altered specificities to both VSCC subtypes. The secondary structures of the three peptides are almost identical, consisting of a triple-stranded beta-sheet and several turns. A comparison of NMR data emphasizes the structural similarities between the peptides and highlights some minor structural differences. In the three-dimensional structures of SVIB and MVIIA these are manifested as orientational differences between two key loops. The structural rigidity of MVIIA was also examined. H alpha shifts are similar in a range of solvents, indicating that there are no solvent-induced changes in structure. The omega-conotoxins form a consensus structure despite differences in sequence and VSCC subtype specificity. This indicates that the omega-conotoxin macrosites for the N/P/Q-subfamily of VSCCs are related, with specificity for receptor targets being conferred by the positions of functional side-chains on the surface of the peptides.
A consensus structure for omega-conotoxins with different selectivities for voltage-sensitive calcium channel subtypes: comparison of MVIIA, SVIB and SNX-202.,Nielsen KJ, Thomas L, Lewis RJ, Alewood PF, Craik DJ J Mol Biol. 1996 Oct 25;263(2):297-310. PMID:8913308[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramilo CA, Zafaralla GC, Nadasdi L, Hammerland LG, Yoshikami D, Gray WR, Kristipati R, Ramachandran J, Miljanich G, Olivera BM, et al.. Novel alpha Biochemistry. 1992 Oct 20;31(41):9919-26. PMID:1390774 doi:10.1021/bi00156a009
- ↑ Nielsen KJ, Thomas L, Lewis RJ, Alewood PF, Craik DJ. A consensus structure for omega-conotoxins with different selectivities for voltage-sensitive calcium channel subtypes: comparison of MVIIA, SVIB and SNX-202. J Mol Biol. 1996 Oct 25;263(2):297-310. PMID:8913308 doi:S0022-2836(96)90576-8
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