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| | <StructureSection load='5anz' size='340' side='right'caption='[[5anz]], [[Resolution|resolution]] 1.61Å' scene=''> | | <StructureSection load='5anz' size='340' side='right'caption='[[5anz]], [[Resolution|resolution]] 1.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5anz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ANZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ANZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5anz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ANZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ANZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ao7|5ao7]], [[5ao8|5ao8]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5anz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5anz OCA], [https://pdbe.org/5anz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5anz RCSB], [https://www.ebi.ac.uk/pdbsum/5anz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5anz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5anz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5anz OCA], [http://pdbe.org/5anz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5anz RCSB], [http://www.ebi.ac.uk/pdbsum/5anz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5anz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HX28_PSEAE Q9HX28_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dominguez-Gil, T]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Hermoso, J A]] | + | [[Category: Dominguez-Gil T]] |
| - | [[Category: Cell wall recycling]] | + | [[Category: Hermoso JA]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Lytic transglycosilase]]
| + | |
| Structural highlights
Function
Q9HX28_PSEAE
Publication Abstract from PubMed
A family of 11 lytic transglycosylases in Pseudomonas aeruginosa, an opportunistic human pathogen, turn over the polymeric bacterial cell wall in the course of its recycling, repair, and maturation. The functions of these enzymes are not fully understood. We disclose herein that SltB3 of P. aeruginosa is an exolytic lytic transglycosylase. We characterize its reaction and its products by the use of peptidoglycan-based molecules. The enzyme recognizes a minimum of four sugars in its substrate but can process a substrate comprised of a peptidoglycan of 20 sugars. The ultimate product of the reaction is N-acetylglucosamine-1,6-anhydro-N-acetylmuramic acid. The X-ray structure of this enzyme is reported for the first time. The enzyme is comprised of four domains, arranged within an annular conformation. The polymeric linear peptidoglycan substrate threads through the opening of the annulus, as it experiences turnover.
Turnover of Bacterial Cell Wall by SltB3, a Multidomain Lytic Transglycosylase of Pseudomonas aeruginosa.,Lee M, Dominguez-Gil T, Hesek D, Mahasenan KV, Lastochkin E, Hermoso JA, Mobashery S ACS Chem Biol. 2016 Jun 17;11(6):1525-31. doi: 10.1021/acschembio.6b00194. Epub, 2016 Apr 11. PMID:27035839[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee M, Dominguez-Gil T, Hesek D, Mahasenan KV, Lastochkin E, Hermoso JA, Mobashery S. Turnover of Bacterial Cell Wall by SltB3, a Multidomain Lytic Transglycosylase of Pseudomonas aeruginosa. ACS Chem Biol. 2016 Jun 17;11(6):1525-31. doi: 10.1021/acschembio.6b00194. Epub, 2016 Apr 11. PMID:27035839 doi:http://dx.doi.org/10.1021/acschembio.6b00194
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