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| | <StructureSection load='5aot' size='340' side='right'caption='[[5aot]], [[Resolution|resolution]] 1.02Å' scene=''> | | <StructureSection load='5aot' size='340' side='right'caption='[[5aot]], [[Resolution|resolution]] 1.02Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5aot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19208 Atcc 19208]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AOT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5AOT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5aot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AOT FirstGlance]. <br> |
| | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5aos|5aos]], [[5aoz|5aoz]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aot OCA], [https://pdbe.org/5aot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aot RCSB], [https://www.ebi.ac.uk/pdbsum/5aot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aot ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5aot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aot OCA], [http://pdbe.org/5aot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aot RCSB], [http://www.ebi.ac.uk/pdbsum/5aot PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aot ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A1A9TAF4_RUMFL A0A1A9TAF4_RUMFL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 19208]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alves, V D]] | + | [[Category: Ruminococcus flavefaciens]] |
| - | [[Category: Bule, P]] | + | [[Category: Alves VD]] |
| - | [[Category: Fernandes, V O]] | + | [[Category: Bule P]] |
| - | [[Category: Ferreira, L M.A]] | + | [[Category: Fernandes VO]] |
| - | [[Category: Fontes, C M.G A]] | + | [[Category: Ferreira LMA]] |
| - | [[Category: Najmudin, S]] | + | [[Category: Fontes CMGA]] |
| - | [[Category: Pires, A J]] | + | [[Category: Najmudin S]] |
| - | [[Category: Pires, V]] | + | [[Category: Pires AJ]] |
| - | [[Category: Ribeiro, T]] | + | [[Category: Pires V]] |
| - | [[Category: Santos, H]] | + | [[Category: Ribeiro T]] |
| - | [[Category: Thompson, A]] | + | [[Category: Santos H]] |
| - | [[Category: Venditto, I]] | + | [[Category: Thompson A]] |
| - | [[Category: Carbohydrate active enzyme]]
| + | [[Category: Venditto I]] |
| - | [[Category: Carbohydrate binding module]]
| + | |
| - | [[Category: Cellulosome]]
| + | |
| - | [[Category: Endoglucanase cel5a]]
| + | |
| - | [[Category: Ruminococcus flavefaciens fd-1]]
| + | |
| - | [[Category: Sugar binding proten]]
| + | |
| Structural highlights
Function
A0A1A9TAF4_RUMFL
Publication Abstract from PubMed
The breakdown of plant cell wall (PCW) glycans is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Defining the portfolio of CBMs, the CBMome, of a PCW degrading system is central to understanding the mechanisms by which microbes depolymerize their target substrates. Ruminococcus flavefaciens, a major PCW degrading bacterium, assembles its catalytic apparatus into a large multienzyme complex, the cellulosome. Significantly, bioinformatic analyses of the R. flavefaciens cellulosome failed to identify a CBM predicted to bind to crystalline cellulose, a key feature of the CBMome of other PCW degrading systems. Here, high throughput screening of 177 protein modules of unknown function was used to determine the complete CBMome of R. flavefaciens The data identified six previously unidentified CBM families that targeted beta-glucans, beta-mannans, and the pectic polysaccharide homogalacturonan. The crystal structures of four CBMs, in conjunction with site-directed mutagenesis, provide insight into the mechanism of ligand recognition. In the CBMs that recognize beta-glucans and beta-mannans, differences in the conformation of conserved aromatic residues had a significant impact on the topology of the ligand binding cleft and thus ligand specificity. A cluster of basic residues in CBM77 confers calcium-independent recognition of homogalacturonan, indicating that the carboxylates of galacturonic acid are key specificity determinants. This report shows that the extended repertoire of proteins in the cellulosome of R. flavefaciens contributes to an extended CBMome that supports efficient PCW degradation in the absence of CBMs that specifically target crystalline cellulose.
Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.,Venditto I, Luis AS, Rydahl M, Schuckel J, Fernandes VO, Vidal-Melgosa S, Bule P, Goyal A, Pires VM, Dourado CG, Ferreira LM, Coutinho PM, Henrissat B, Knox JP, Basle A, Najmudin S, Gilbert HJ, Willats WG, Fontes CM Proc Natl Acad Sci U S A. 2016 Jun 13. pii: 201601558. PMID:27298375[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Venditto I, Luis AS, Rydahl M, Schuckel J, Fernandes VO, Vidal-Melgosa S, Bule P, Goyal A, Pires VM, Dourado CG, Ferreira LM, Coutinho PM, Henrissat B, Knox JP, Basle A, Najmudin S, Gilbert HJ, Willats WG, Fontes CM. Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition. Proc Natl Acad Sci U S A. 2016 Jun 13. pii: 201601558. PMID:27298375 doi:http://dx.doi.org/10.1073/pnas.1601558113
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