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| | <StructureSection load='5azo' size='340' side='right'caption='[[5azo]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5azo' size='340' side='right'caption='[[5azo]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5azo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AZO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5azo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AZO FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5azp|5azp]], [[5azs|5azs]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azo OCA], [https://pdbe.org/5azo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5azo RCSB], [https://www.ebi.ac.uk/pdbsum/5azo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5azo ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">oprN, PA2495 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azo OCA], [http://pdbe.org/5azo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5azo RCSB], [http://www.ebi.ac.uk/pdbsum/5azo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5azo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9I0Y7_PSEAE Q9I0Y7_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Nakagawa, A]] | + | [[Category: Nakagawa A]] |
| - | [[Category: Yamashita, E]] | + | [[Category: Yamashita E]] |
| - | [[Category: Yonehara, R]] | + | [[Category: Yonehara R]] |
| - | [[Category: Alpha barrel]]
| + | |
| - | [[Category: Beta barrel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
Q9I0Y7_PSEAE
Publication Abstract from PubMed
The genome of Pseudomonas aeruginosa encodes tripartite efflux pumps that extrude functionally and structurally dissimilar antibiotics from the bacterial cell. MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM are the main tripartite efflux pumps responsible for multidrug resistance in P. aeruginosa. The outer membrane factors OprN, OprJ, and OprM are essential components of functional tripartite efflux pumps. To elucidate the structural basis of multidrug resistance, we determined the crystal structures of OprN and OprJ. These structures revealed several features, including tri-acylation of the N-terminal cysteine, a small pore in the beta-barrel domain, and a tightly sealed gate in the alpha-barrel domain. Despite the overall similarity of OprN, OprJ, and OprM, a comparison of their structures and electrostatic distributions revealed subtle differences at the periplasmic end of the alpha-barrel domain. These results suggested that the overall structures of these outer membrane factors are specifically optimized for particular tripartite efflux pumps. Proteins 2016; 84:759-769. (c) 2016 Wiley Periodicals, Inc.
Crystal structures of OprN and OprJ, outer membrane factors of multidrug tripartite efflux pumps of Pseudomonas aeruginosa.,Yonehara R, Yamashita E, Nakagawa A Proteins. 2016 Jun;84(6):759-69. doi: 10.1002/prot.25022. Epub 2016 Mar 9. PMID:26914226[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yonehara R, Yamashita E, Nakagawa A. Crystal structures of OprN and OprJ, outer membrane factors of multidrug tripartite efflux pumps of Pseudomonas aeruginosa. Proteins. 2016 Jun;84(6):759-69. doi: 10.1002/prot.25022. Epub 2016 Mar 9. PMID:26914226 doi:http://dx.doi.org/10.1002/prot.25022
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