This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
8gsx
From Proteopedia
(Difference between revisions)
m (Protected "8gsx" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==X-ray structure of Clostridium perfringens pili protein B collagen-binding domains== | |
| + | <StructureSection load='8gsx' size='340' side='right'caption='[[8gsx]], [[Resolution|resolution]] 2.86Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8gsx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_str._13 Clostridium perfringens str. 13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GSX FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gsx OCA], [https://pdbe.org/8gsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gsx RCSB], [https://www.ebi.ac.uk/pdbsum/8gsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gsx ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8XP11_CLOPE Q8XP11_CLOPE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sortase-mediated pili are flexible rod proteins composed of major and minor/tip pilins, playing important roles in the initial adhesion of bacterial cells to host tissues. The pilus shaft is formed by covalent polymerization of major pilins, and the minor/tip pilin is covalently attached to the tip of the shaft involved in adhesion to the host cell. The Gram-positive bacterium Clostridium perfringens has a major pilin, and a minor/tip pilin (CppB) with the collagen-binding motif. Here, we report X-ray structures of CppB collagen-binding domains, collagen-binding assays and mutagenesis analysis, demonstrating that CppB collagen-binding domains adopt an L-shaped structure in open form, and that a small beta-sheet unique to CppB provides a scaffold for a favourable binding site for collagen peptide. | ||
| - | + | Structural and biochemical characterization of Clostridium perfringens pili protein B collagen-binding domains.,Tamai E, Yamada M, Ishida T, Arimura N, Matsunami R, Sekiya H, Kamitori S FEBS Lett. 2023 May;597(10):1345-1354. doi: 10.1002/1873-3468.14626. Epub 2023 , Apr 27. PMID:37071018<ref>PMID:37071018</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8gsx" style="background-color:#fffaf0;"></div> |
| - | [[Category: Tamai | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Clostridium perfringens str. 13]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kamitori S]] | ||
| + | [[Category: Tamai E]] | ||
| + | [[Category: Yamada M]] | ||
Revision as of 05:42, 7 June 2023
X-ray structure of Clostridium perfringens pili protein B collagen-binding domains
| |||||||||||
